Native Alcaligenes sp. Choline Oxidase
| Cat# | DIA-184 |
| Source | Alcaligenes sp. |
| Description | In enzymology, a choline oxidase (EC 1.1.3.17) is an enzyme that catalyzes the chemical reaction: choline + O2? betaine aldehyde + H2O2. Thus, the two substrates of this enzyme are choline and O2, whereas its two products are betaine aldehyde and H2O2. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with oxygen as acceptor. |
| Form | Freeze dried powder |
| Activity | GradeIII 10U/mg-solid or more (containing approx. 20% of stabilizers) |
| CAS No. | 9028-67-5 |
| Isoelectric point | 4.1±0.1 |
| Synonyms | choline oxidase; EC 1.1.3.17 |
| Enzyme Commission Number | EC 1.1.3.17 |
| pH Stability | pH 7.0-9.0 (30°C, 2 hr) |
| Michaelis Constant | 2.84×10?3M (Choline), 5.33×10?3M(Betaine aldehyde) |
| Optimum pH | 8.0-8.5 |
| Optimum temperature | 40-45°C |
| Thermal stability | below 37°C (pH 7.5, 10min) |
| Stability | Stable at-20°C for at least 6 months |
| Stabilizers | EDTA, bovine serum albumin, amino acids (glycine, sodium gluta-mate, etc.) |
| Inhibitors | p-Chloromercuribenzoate, Cu??, Co??, Hg??, Ag? |
| Contaminants | Catalase < 1.0×102% |
| Abbr | Choline Oxidase (Alcaligenes sp.) |
| Applications | This enzyme is useful for enzymatic determination of phospholipids when coupled with phospholipase D and for choline esterase-activity in clinical analysis. |
| Appearance | Yellowish amorphous powder, lyophilized |
| Structure | One mol of FAD is covalently bound to mol of the enzyme |
| Molecular Weight | approx. 95 kDa |
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