| Cat# | DIA-454 |
| Description | Engineered large fragment of Bacillus stearothermophilus DNA polymerase. Retains 5′→3′ polymerase and strong strand displacement activity, lacks 5′→3′ exonuclease activity. Incorporates next-generation hot-start technology: inhibited below 50 °C, rapidly activated above 50 °C. Suitable for isothermal amplification with high speed, specificity, salt tolerance, and thermal stability. Supports room-temperature reaction preparation. |
| Storage | −30 °C to −15 °C |
| Applications | Isothermal amplification: LAMP (Loop-Mediated Isothermal Amplification), HDA (Helicase-Dependent Amplification), RCA (Rolling Circle Amplification). |
| Product Overview | Product Components: 1600 U: Bst II Pro DNA Polymerase Large Fragment (8 U/μL): 200 μL 10x lsothermalAmp Buffer: 500 μL MgSO4(100 mM): 300 μL 8000 U: Bst II Pro DNA Polymerase Large Fragment (8 U/μL): 1 mL 10x lsothermalAmp Buffer: 3 x 1 mL MgSO4(100 mM): 2 x 1 mL Product Features: Excellent amplification performance Compatible with crude lysate samples Supports room temperature system setup |
| Package | 1600/8000 U |
| Download Datasheet: |  |
