| Cat# | DIA-534 |
| Source | Bacillus subtilis |
| Description | High purity lichenase (endo-1,3,1,4-β-glucanase) (Bacillus subtilis) for use in research, biochemical enzyme assays and in vitro diagnostic analysis. |
| Form | Suspension |
| ECNumber | 3.2.1.73 |
| Activity | ~ 230 U/mg (40 °C, pH 6.5 on barley β-glucan) |
| CAS No. | 37288-51-0 |
| Optimum temperature | 60 °C |
| Stability | > 1 year under recommended storage conditions |
| Unit Definition | One unit of lichenase activity is defined as the amount of enzyme required to release one μmole of glucose reducing-sugar equivalents per minute from barley β-glucan (10 mg/mL) in sodium phosphate buffer (100 mM), pH 6.5 at 40 °C. |
| Storage | 2–8 °C |
| Synonyms | Licheninase; (1→3)-(1→4)-β-D-glucan 4-glucanohydrolase |
| Buffer | 3.2 M ammonium sulphate |
| Applications | Applications in carbohydrate research and in the food and feeds, brewing and biofuels industries. |
| Molecular Weight | 26750 Da |
| Concentration | ~ 1000 U/mL |
| Specificity | Hydrolysis of (1,4)-β-D-glucosidic linkages in β-D-glucans containing (1,3)- and (1,4)-bonds. |
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