| Cat# | ENZD-RT12 |
| Specification | 200 rxns 1000 rxns 10000 rxns |
| Aplications | Rapid Detection; Virus Detection; Pathogen Detection; Fusion Gene Detection; DNA/RNA Co-Detection; Gene Expression Analysis; Drug Residue Detection; Low Copy Number Gene Detection |
| Description | For rapid one-step RT-qPCR detection of RNA templates, using antibody-modified Taq enzyme and high-performance reverse transcriptase to achieve rapid and efficient amplification. Contains a dUTP/UDG anti-contamination system, suitable for single or multiple detection, with accurate and reliable results. |
| Features | 1. Rapid amplification: Able to achieve 30-minute amplification, improving experimental efficiency. 2.Compatible with tolerance to impurities: Compatible with crude lysis solution of nasopharyngeal swab samples. 3. Sensitive and efficient: Able to stably detect low concentration samples. · 4. DNA/RNA co-detection: Able to stably achieve balanced amplification of DNA and RNA templates. |
| Quality Control | Achieves fast amplification within 30 min Sample compatibility Stable detection at low concentrations |
| Components | RNase-free ddH2O 5 × One Step U+ Mix One Step U+ Enzyme Mix 50 × ROX Reference Dye 1 50 × ROX Reference Dye 2 |
| Shipping and Storage Conditions | Store at -30 ~ -15℃, transport at ≤0℃. |
| Download Datasheet: |  |
The ENZD-RT12 one-step RT-qPCR probe kit from Creative Enzymes was optimized for fast cycling conditions and evaluated for rapid amplification performance.
Under accelerated reaction conditions, ENZD-RT12 achieved RT-qPCR amplification within 30 minutes, while maintaining consistent performance between fast and standard protocols. CT variation remained within ±1 cycle and plateau differences within 10%, indicating stable kinetics under rapid workflows. The system is built on a hot-start DNA Polymerase formulation and incorporates a dUTP/UDG contamination prevention system to ensure high specificity during fast amplification. Data sourced from internal validation studies evaluating rapid RT-qPCR performance under fast cycling conditions.
ENZD-RT12 was evaluated using different biological matrices, including fecal and tissue-derived samples, and compared with commercial RT-qPCR reagents.
The results showed consistently lower CT values across multiple sample types, indicating improved sensitivity and robust performance in complex biological backgrounds. Data sourced from internal validation studies assessing RT-qPCR performance across diverse sample matrices.
Low-concentration pseudovirus samples were used to assess detection sensitivity of ENZD-RT12 in multiplex RT-qPCR systems.
Across 32 replicates in two assay formats, the detection rate exceeded 95%, demonstrating strong sensitivity and stable performance at low template input levels. Data sourced from internal validation studies evaluating low-copy detection performance in multiplex RT-qPCR systems.
