| Cat# | Kit-060 |
| Description | This DNA library preparation kit is specifically optimized for the Illumina high-throughput sequencing platform. It converts 100 pg–1 μg of fragmented double-stranded input DNA into sequencing-ready libraries compatible with Illumina systems. The upgraded version incorporates optimized end-repair, adapter ligation, and library amplification modules, significantly improving library conversion rates for low-quality templates and reducing duplication rates. It is broadly applicable for PCR and PCR-free library construction and is compatible with targeted capture workflows. All reagents are rigorously quality-controlled and functionally validated to ensure stable and reproducible library preparation. |
| Storage | -30 °C to -15 °C |
| Applications | Whole-genome sequencing (WGS); whole-exome sequencing (WES) or other targeted capture sequencing; amplicon sequencing; chromatin immunoprecipitation sequencing (ChIP-seq); metagenomic sequencing; methylation sequencing. |
| Product Overview | Broad sample compatibility: Compatible not only with conventional samples, but also with low-quality FFPE and cfDNA samples. High library construction efficiency: Delivers higher library conversion rates. Accurate mutation detection: Enables reliable detection of low-frequency variants. |
| Package | 24 rxns End Prep Buffer 2: 168 μL End Prep Enzyme 2: 72 μL Rapid Ligation Buffer 5: 720 μL Rapid DNA Ligase 2: 240 μL HiFi Amplification Mix 2: 600 μL PCR Primer Mix 3 for Illumina: 120 μL Control DNA (264 bp, 50 ng/μL): 10 μL DNA Reagents: 96 rxns (End Prep Buffer 2: 130 μL each; End Prep Enzyme 2: 60 μL each; Rapic Ligation Buffer 5: 420 μL each; Rapid DNA Ligase 2: 150 μL each; HiFi Amplification Mix 2: 350 μL each; PCR Primer Mix 3 for Illumina: 80 μL each) |
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