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High II U+ One Step qRT-PCR Probe Kit

Cat# ENZD-RT15
Specification 100 rxns (30 μl/rxn)
5,000 rxns (30 μl/rxn)
Aplications Virus Detection; Pathogen Detection; Gene Expression Analysis
Description Designed specifically for RNA quantitative PCR, with a detection sensitivity of up to 0.1 pg total RNA or <10 copies of RNA. Uses a Heat-labile UDG anti-contamination system and 2× Master Mix format, compatible with TaqMan and other probe-based detection methods.
Features 1. Excellent amplification sensitivity: The superior performance of the integrated High II Reverse Transcriptase and heat-start Champagne Taq DNA Polymerase, combined with an optimized buffer system, enhances detection sensitivity.
2. UDG anti-contamination system: Heat-labile UDG can rapidly degrade U-containing contaminants at room temperature; at 55°C during reverse transcription, Heat-labile UDG quickly inactivates, not affecting the efficiency and sensitivity of qRT-PCR.
3. Supports fast protocols: Compatible with fast protocols to improve detection efficiency.\n· Compatible with multiple probe detection.
Quality Control Excellent amplification sensitivity
Excellent compatibility with multiple systems
Components RNase-free ddH2O
2 × One Step U+ Mix
One Step U+ Enzyme Mix
50 × ROX Reference Dye 1
50 × ROX Reference Dye 2
Shipping and Storage Conditions Store at -30 ~ -15℃, transport at ≤0℃.
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For research and industrial use only, not for personal medicinal use.

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