| Cat# | ENZD-RT15 |
| Specification | 100 rxns (30 μl/rxn) 5,000 rxns (30 μl/rxn) |
| Aplications | Virus Detection; Pathogen Detection; Gene Expression Analysis |
| Description | Designed specifically for RNA quantitative PCR, with a detection sensitivity of up to 0.1 pg total RNA or <10 copies of RNA. Uses a Heat-labile UDG anti-contamination system and 2× Master Mix format, compatible with TaqMan and other probe-based detection methods. |
| Features | 1. Excellent amplification sensitivity: The superior performance of the integrated High II Reverse Transcriptase and heat-start Champagne Taq DNA Polymerase, combined with an optimized buffer system, enhances detection sensitivity. 2. UDG anti-contamination system: Heat-labile UDG can rapidly degrade U-containing contaminants at room temperature; at 55°C during reverse transcription, Heat-labile UDG quickly inactivates, not affecting the efficiency and sensitivity of qRT-PCR. 3. Supports fast protocols: Compatible with fast protocols to improve detection efficiency.\n· Compatible with multiple probe detection. |
| Quality Control | Excellent amplification sensitivity Excellent compatibility with multiple systems |
| Components | RNase-free ddH2O 2 × One Step U+ Mix One Step U+ Enzyme Mix 50 × ROX Reference Dye 1 50 × ROX Reference Dye 2 |
| Shipping and Storage Conditions | Store at -30 ~ -15℃, transport at ≤0℃. |
| Download Datasheet: |  |
The ENZD-RT15 one-step RT-qPCR probe kit from Creative Enzymes was evaluated across three independent viral detection systems and compared with commercial probe-based qRT-PCR reagents under identical conditions.
ENZD-RT15 demonstrated higher amplification sensitivity and stronger signal plateau across all systems, indicating improved detection capability for low-abundance targetse in Multiplex Assays
To assess multiplex capability, ENZD-RT15 was tested in both triplex and quadruplex viral detection systems.
The results showed stable amplification across all targets, with no significant loss in efficiency or sensitivity compared to singleplex reactions. This demonstrates reliable multiplex compatibility and balanced signal output in complex assay formats. Data sourced from internal validation studies evaluating multiplex amplification performance in viral detection systems.
