Native Microorganism Glucose Dehyrogenase (NAD(P)-dependent)
| Cat# | DIA-191 |
| Abbr | Glucose Dehyrogenase (Microorganism) |
| Source | Microorganism |
| Description | In enzymology, a glucose 1-dehydrogenase (EC 1.1.1.47) is an enzyme that catalyzes the chemical reaction: beta-D-glucose + NAD(P)+ ↔ D-glucono-1,5-lactone + NAD(P)H + H+. The 3 substrates of this enzyme are beta-D-glucose, NAD+, and NADP+, whereas its 4 products are D-glucono-1,5-lactone, NADH, NADPH, and H+. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor. |
| Applications | This enzyme is useful for enzymatic determination of D-Glucose. |
| Appearance | White amorphous powder, lyophilized |
| Form | Freeze dried powder |
| Enzyme Commission Number | EC 1.1.1.47 |
| Activity | 250U/mg-solid or more |
| CAS No. | 9028-53-9 |
| Contaminants | NADH oxidase < 1.0×10⁻³% α-Glucosidase < 1.0×10⁻³% Glucose-6-phosphate dehydrogenase < 1.0×10⁻³% |
| Molecular Weight | approx. 101 kDa (Gel filtration) |
| Isoelectric point | 4.5 |
| pH Stability | pH 6.0-7.5 (20°C, 16hr) |
| Michaelis Constant | NAD⁺linked : 1.38×10⁻²M (D-Glucose) 3.09×10⁻⁴M (NAD⁺), NADP⁺linked : 1.25×10⁻²M (D-Glucose) 4.07×10⁻⁵M (NADP⁺) |
| Specificity | Specific for ß-D,-Glucose or 2-Deoxy-glucose (Either NAD⁺ or NADP⁺ serves as coenzyme.) |
| Optimum pH | 9 |
| Optimum temperature | 55°C |
| Thermal stability | 45°C (15min-treatment with 50mM K-phosphate buffer, pH 7.0) |
| Stability | Stable at-20°C for at least one year |
| Inhibitors | Ag⁺, Hg²⁺, Monoiodoacetate |
| Synonyms | Glucose Dehyrogenase; EC 1.1.1.47; beta-D-glucose: NAD(P)+ 1-oxidoreductase; D-glucose dehydrogenase (NAD(P)+) |
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