diagnostic-enzymes

Native Microorganism Glucose Dehyrogenase (NAD(P)-dependent)

Native Microorganism Glucose Dehyrogenase (NAD(P)-dependent)

Cat# AbbrSimilarSourceDescriptionApplicationsAppearanceFormEnzyme Commission NumberActivityCAS No. ContaminantsMolecular WeightIsoelectric pointpH StabilityMichaelis ConstantSpecificityOptimum pHOptimum temperatureThermal stabilityStabilityInhibitorsSynonyms
DIA-191 Glucose Dehyrogenase (Microorganism)Glucose DehyrogenaseMicroorganismIn enzymology, a glucose 1-dehydrogenase (EC 1.1.1.47) is an enzyme that catalyzes the chemical reaction: beta-D-glucose + NAD(P)+ ↔ D-glucono-1,5-lactone + NAD(P)H + H+. The 3 substrates of this enzyme are beta-D-glucose, NAD+, and NADP+, whereas its 4 products are D-glucono-1,5-lactone, NADH, NADPH, and H+. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor.This enzyme is useful for enzymatic determination of D-Glucose.White amorphous powder, lyophilizedFreeze dried powderEC 1.1.1.47250U/mg-solid or more9028-53-9NADH oxidase < 1.0×10⁻³% α-Glucosidase < 1.0×10⁻³% Glucose-6-phosphate dehydrogenase < 1.0×10⁻³%approx. 101 kDa (Gel filtration)4.5pH 6.0-7.5 (20°C, 16hr)NAD⁺linked : 1.38×10⁻²M (D-Glucose) 3.09×10⁻⁴M (NAD⁺), NADP⁺linked : 1.25×10⁻²M (D-Glucose) 4.07×10⁻⁵M (NADP⁺)Specific for ß-D,-Glucose or 2-Deoxy-glucose (Either NAD⁺ or NADP⁺ serves as coenzyme.)955°C45°C (15min-treatment with 50mM K-phosphate buffer, pH 7.0)Stable at-20°C for at least one yearAg⁺, Hg²⁺, MonoiodoacetateGlucose Dehyrogenase; EC 1.1.1.47; beta-D-glucose: NAD(P)+ 1-oxidoreductase; D-glucose dehydrogenase (NAD(P)+) Download Datasheet:
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