| Cat# | DIA-582 |
| Source | Escherichia coli |
| Description | The Klenow fragment is a proteolytic product of E. coli DNA polymerase I. It retains 5′→3′ DNA polymerase activity and 3′→5′ exonuclease (proofreading) activity but lacks 5′→3′ exonuclease activity. This enzyme maintains high fidelity while avoiding degradation of DNA 5′ ends, making it suitable for fill-in reactions and second-strand synthesis. |
| Unit Definition | One unit is defined as the amount of enzyme required to incorporate 10 nmol of dNTP into acid-insoluble material within 30 minutes at 37 °C. |
| Storage | -20 °C |
| Buffer | 25 mM Tris-HCl pH 7.4, 0.1 mM EDTA, 1 mM DTT, 50% glycerol |
| Applications | Sanger dideoxy DNA sequencing; removal of 3′ overhangs or filling-in of 5′ overhangs to generate blunt ends; second-strand cDNA synthesis; end repair of inserts during library preparation. |
| Product Overview | Probe preparation using random primers. Removal of 3′ overhangs or filling in of 5′ overhangs to generate blunt ends. |
| Package | 5000 U Klenow Fragment (5 U/μL): 1 mL 10× Blue Buffer (100 mM Tris-HCl pH 7.9, 500 mM NaCl, 100 mM MgCl2, 10 mM DTT): 2 mL |
| Download Datasheet: |  |
