| Cat# | Kit-071 |
| Description | This RNA-seq library preparation kit is optimized for Illumina high-throughput sequencing platforms. It includes two types of second-strand cDNA synthesis buffers, enabling construction of either standard or strand-specific transcriptome libraries. Second-strand synthesis, end repair, and dA-tailing are combined into a single step without intermediate purification, simplifying the workflow and reducing library preparation time. An optimized reaction system improves library conversion efficiency, supports lower RNA input amounts, and provides uniform coverage across a wide range of input levels. |
| Storage | -30 °C to -15 °C |
| Applications | RNA library preparation; gene expression analysis; single nucleotide variant (SNV) analysis; alternative splicing analysis; fusion gene detection; targeted transcriptome analysis. |
| Product Overview | High library yield: Upgraded workflow efficiency (reverse transcription, nucleic acid repair, adapter ligation, library amplification); compatible with multiple species. High sequencing quality: High mRNA proportion and excellent downstream data. |
| Package | 24 rxns Frag/Prime Buffer 2: 480 μL 1st Strand Buffer 6: 168 μL 1st Strand Enzyme Mix 4: 48 μL 2nd Strand Buffer 3 (with dNTP): 600 μL 2nd Strand Enzyme Super Mix 3: 360 μL Rapid Ligation Buffer 6: 600 μL Rapid DNA Ligase 6: 120 μL PCR Primer Mix 5: 120 μL HiFi Amplification Mix 3: 600 μL |
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