| Cat# | ENZD-MDLyo05 |
| Reaction | 200 rxns; 500 rxns |
| Description | The LytoReady™ U+ One Step RT-qPCR Probe Kit is a glycerol-free, lyophilized reagent for detecting RNA templates, optimized for use with appropriate lyophilization protocols. Development of lyophilized reagents typically presents challenges in stability, lyophilization yield, and long-term storage. This kit overcomes these issues through optimized functional proteins, excipient selection, and lyophilization processes. By using highly stable enzymes and protectants, the kit achieves storage stability comparable to glycerol-containing reagents. Furthermore, optimized excipients and processes improve lyophilization yield and enable room-temperature storage for over a year. The kit retains the advantages of conventional reagents, including excellent multiplex amplification, specificity, and sensitivity. Its integrated dUTP/UDG system prevents contamination, ensuring accurate qPCR results. |
| Notes | Glycerin-free, one-step RT-qPCR premix. |
| Features | Excellent post-freezing stability: It can achieve long-term stability after 60 days of pressurization at 50 ℃ and 360 days at 25 ℃. Sensitive and efficient: It has excellent amplification performance. Rapid amplification: Enables amplification in 27 minutes, improving experimental efficiency. |
| Components | RNase-free ddH2O 5 × One Step Mix One Step Lyo-Enzyme Mix 3 × Excipient 50 × ROX Reference Dye 1 50 × ROX Reference Dye 2 |
| Quality Control | Stable for long-term storage after freezing; Excellent amplification performance |
| Shipping and Storage Conditions | Store at -30 ~ -15℃, transport at ≤0℃. |
| Download Datasheet: |  |
ENZD-MDLyo05 from Creative Enzymes was evaluated in a lyophilized multiplex respiratory detection system. After freeze-drying, the reagents were vacuum-sealed in aluminum foil packaging and stored under accelerated and long-term conditions.
Performance was assessed after storage at 50°C for 60 days and 25°C for up to one year, using liquid-form reagents as the reference. Results showed no significant difference in amplification performance between lyophilized and liquid formulations under all tested conditions. Data generated from internal validation studies, confirming consistent amplification performance after long-term storage under elevated and ambient temperatures.
The amplification performance of ENZD-MDLyo05 was evaluated across different reaction systems and compared with glycerol-free one-step reagents from other commercial suppliers under identical primer–probe conditions.
Results showed that ENZD-MDLyo05 consistently achieved lower CT values, indicating higher amplification sensitivity across all tested systems. In addition, the enzyme demonstrated high specificity with stable signal output, supporting accurate and reliable detection.
These results highlight the strong multiplex amplification performance of ENZD-MDLyo05, making it well-suited for multi-target detection workflows requiring both high sensitivity and high specificity. Data generated from internal validation studies, demonstrating enhanced amplification sensitivity and consistent performance across multiple reaction systems.
