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LytoReady™ U+ One Step RT-qPCR Probe Kit (Glycerol-free)

Cat# ENZD-MDLyo05
Reaction 200 rxns; 500 rxns
Description The LytoReady™ U+ One Step RT-qPCR Probe Kit is a glycerol-free, lyophilized reagent for detecting RNA templates, optimized for use with appropriate lyophilization protocols. Development of lyophilized reagents typically presents challenges in stability, lyophilization yield, and long-term storage. This kit overcomes these issues through optimized functional proteins, excipient selection, and lyophilization processes. By using highly stable enzymes and protectants, the kit achieves storage stability comparable to glycerol-containing reagents. Furthermore, optimized excipients and processes improve lyophilization yield and enable room-temperature storage for over a year. The kit retains the advantages of conventional reagents, including excellent multiplex amplification, specificity, and sensitivity. Its integrated dUTP/UDG system prevents contamination, ensuring accurate qPCR results.
Notes Glycerin-free, one-step RT-qPCR premix.
Features Excellent post-freezing stability: It can achieve long-term stability after 60 days of pressurization at 50 ℃ and 360 days at 25 ℃.
Sensitive and efficient: It has excellent amplification performance.
Rapid amplification: Enables amplification in 27 minutes, improving experimental efficiency.
Components RNase-free ddH2O
5 × One Step Mix
One Step Lyo-Enzyme Mix
3 × Excipient
50 × ROX Reference Dye 1
50 × ROX Reference Dye 2
Quality Control Stable for long-term storage after freezing;
Excellent amplification performance
Shipping and Storage Conditions Store at -30 ~ -15℃, transport at ≤0℃.
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