| Cat# | ENZD-MDLyo06 |
| Reaction | 200 rxns; 1,000 rxns; 5,000 rxns |
| Description | The AdvSTART U+ One Step RT-qPCR Probe Kit (Glycerol-free) is a lyophilization-compatible, glycerol-free, one-step RT-qPCR reagent designed for RNA templates like RNA viruses. Combining a high-performance Reverse Transcriptase and hot-start Champagne Taq DNA Polymerase with a tailored chemical buffer, it delivers exceptional amplification efficiency, uniformity, and specificity. The included dUTP/UDG system prevents contamination at room temperature, ensuring accurate qPCR results. |
| Notes | Advanced AccurSTART glycerol-free one-step RT-qPCR premix |
| Features | • Sensitive and efficient: Excellent amplification performance • Storage stability: Excellent stability under pressure and freeze-thaw cycles • Compatible with a wide variety of samples: compatible with various types of samples including feces, tissues, and swabs. |
| Applications | Virus detection; pathogen detection; fusion gene detection; gene expression analysis |
| Components | RNase-free ddH2O 5 x One Step U+ Mix One Step U+ Enzyme Mix (Glycerol-free) 50 × ROX Reference Dye 1 50 × ROX Reference Dye 2 |
| Quality Control | 1. Excellent amplification performance 2. Stable storage |
| Shipping and Storage Conditions | Store at -30 ~ -15℃, transport at ≤0℃. |
| Download Datasheet: |  |
ENZD-MDLyo06 was evaluated in multiple reaction systems and compared with a commercial glycerol-free one-step amplification reagent under identical primer-probe conditions. The assay was performed using different template matrices to assess overall amplification efficiency. As a high-performance Reverse Transcriptase and hot-start Champagne Taq DNA Polymerase-based system, ENZD-MDLyo06 demonstrated consistently stronger amplification performance across all tested systems compared with the reference reagent, showing improved signal generation and overall reaction efficiency under equivalent conditions. Data sourced from internal validation studies, supporting the consistency and purity of enzyme performance.
ENZD-MDLyo06 was subjected to stability evaluation under different storage temperatures and freeze-thaw conditions, with -20°C storage as the reference control. Amplification performance was assessed by Ct value comparison across test and control groups. Results showed that the Ct variation between tested samples and control remained within ±0.5 cycles, indicating minimal performance drift. These findings demonstrate that ENZD-MDLyo06, incorporating a high-performance Reverse Transcriptase and hot-start Champagne Taq DNA Polymerase, maintains excellent stability under both thermal and mechanical stress conditions. Data sourced from internal validation studies, confirming stable performance and high purity.
