| Cat# | ENZD-QM04 |
| Specification | 200 rxns 500 rxns 2500 rxns |
| Aplications | Multiplex Quantification; Diagnostic Pathogen Detection; Animal Pathogen Detection; Biomedical Quality Control Verification |
| Description | Suitable for DNA template multiplex probe qPCR amplification, with high sensitivity, strong specificity, and good tolerance to complex samples. It has an integrated dUTP/UDG anti-contamination system, supports fast programs and primer-probe premixing, and is an all-around 2× premix solution. |
| Features | 1. High sensitivity: New hot-start Taq enzyme & upgraded buffer system achieve high-sensitivity detection, effectively reducing the rate of missed detections. 2. Wide GC compatibility: Broadly compatible with systems containing 20% to 75% GC content, supporting simultaneous detection of multiple targets. 3. Convenient and efficient use: Supports premixed primer-probe systems with stable performance after 37°C pressurization for 28 days and 50 cycles of freeze-thaw, reducing operational complexity and improving experimental success rates. 4. Supports fast protocols: Detection can be completed within 30 minutes, effectively improving detection efficiency. 5. dUTP/UDG anti-contamination system: Effective at room temperature, reducing false positives and ensuring the authenticity and reliability of results. |
| Quality Control | 1. Excellent sensitivity 2. Supports fast protocols |
| Components | 2 × All-Powerful qPCR PreMix(20 µl/run), including dNTP/dUTP Mix, Mg²⁺, EnzSmart DNA Polymerase, Heat-labile UDG, etc. |
| Shipping and Storage Conditions | Store at -30 ~ -15℃, transport at ≤0℃ |
| Download Datasheet: |  |
The analytical sensitivity of ENZD-QM04 was assessed using a DNA virus template, in parallel with a comparable commercial reagent (Supplier B) under identical reaction conditions.
ENZD-QM04, a novel hot-start Taq DNA polymerase developed by Creative Enzymes, demonstrated improved detection sensitivity along with a higher fluorescence plateau compared to equivalent products. These results indicate more efficient target amplification and stronger signal generation, supporting reliable detection in low-template scenarios. Data sourced from internal validation studies evaluating detection sensitivity and amplification signal intensity using DNA virus templates.
To evaluate compatibility with accelerated workflows, ENZD-QM04 was tested under both standard and rapid qPCR cycling conditions. In addition, its performance under rapid protocols was benchmarked against Supplier A and Supplier B.
The results showed that ENZD-QM04 maintained efficient amplification under rapid cycling conditions, with performance comparable to or exceeding that of other tested reagents. This demonstrates its suitability for time-sensitive detection workflows. Data sourced from internal validation studies assessing amplification efficiency under standard and rapid qPCR cycling protocols.
