| Cat# | ENZD-QM05 |
| Specification | 200 rxns 500 rxns 5000 rxns |
| Aplications | Multiplex Quantification; Gene Expression Analysis; Diagnostic Pathogen Detection; Tumor Detection |
| Description | Suitable for DNA template multiplex probe qPCR amplification, supporting primer-probe premixing. Using high-specificity enzyme and optimized buffer system, it enhances the stability of the multiplex system and has an integrated dUTP/UDG anti-contamination system, effectively preventing aerosol contamination. It is provided in a 5× Mix form, which is convenient to operate. |
| Features | 1. Convenient and efficient use: Supports fully premixed primer-probe systems, stable performance after 37°C pressurization for 28 days and 50 cycles of freeze-thaw, reducing operational complexity and improving experimental success rates. 2. High specificity: Uses dual-species antibody封闭Taq enzyme, largely avoiding non-specific amplification caused by primer-dimer interactions, maintaining a high plateau phase even in systems with 10 or more targets. 3. Wide GC compatibility: Broadly compatible with systems containing 20% to 75% GC content. 4. dUTP/UDG anti-contamination system: Effective at room temperature, reducing false positives and ensuring the authenticity and reliability of results. 5. Large optimization space: Provides split versions for convenient system adjustment and optimization. |
| Quality Control | Excellent premix stability Higher specificity in multiple systems |
| Components | 5 × Super Multiple qPCR PreMix(20 µl/run), including dNTP/dUTP Mix, Mg²⁺, EnzSmart DNA Polymerase, Heat-labile UDG, etc. |
| Shipping and Storage Conditions | Store at -30 ~ -15℃, transport at ≤0℃ |
| Download Datasheet: |  |
The stability of ENZD-QM05 was investigated after pre-mixing with primers and probes, followed by exposure to various stress conditions, including storage at 4°C for 4 months, incubation at 37°C for 28 days, and 50 repeated freeze–thaw cycles. A premix stored at -20°C was used as the reference.
Across all tested conditions, ENZD-QM05 maintained highly consistent amplification performance, with Ct variation within ±0.5 and plateau signal differences within 20% relative to the control. This stability is supported by its integrated dUTP/UDG contamination control system and optimized enzyme formulation. Data sourced from internal validation studies evaluating premix stability under extended storage, thermal stress, and repeated freeze–thaw conditions.
ENZD-QM05 utilizes a dual-antibody hot-start Taq polymerase designed to achieve strong enzyme inhibition prior to activation, enabling precise target amplification even in complex multiplex environments.
Its specificity was evaluated in a high-multiplex HPV detection system (15-plex), in comparison with Supplier A. Despite the presence of multiple primer–probe sets, ENZD-QM05 demonstrated selective amplification of target sequences with improved plateau performance, indicating reduced non-specific amplification and enhanced reaction fidelity. Data sourced from internal validation studies assessing amplification specificity and performance in high-multiplex PCR systems.
