| Cat# | POL-006 |
| Notes | Please use RNasefree supplies during the experiment. For your safety and health, please wear a lab coat and disposable gloves. |
| Storage | Store at -25 ~ -15°C for 2 years. |
| Activity | 20 U/μL |
| Features | E. coli genomic DNA residue < 0.02 copies/100 U, maintaining the lowest level in the industry. Suitable for the development of fully premixed systems: Validation of multi-primer probe systems; verification of fully premixed stability at 37°C for 7 days, highly versatile. High-sensitivity system development: Cleaner enzyme raw materials reduce sequence complexity in the system, improving the sensitivity of detection reagents. |
| Description | Advanced Hotstart Taq DNA Polymerase is a hot-start DNA polymerase that uses dual antibodies for double blocking. It blocks not only the 5′→3′ polymerase activity of Taq DNA polymerase but also the 5′→3′ exonuclease activity. Heating at the pre-denaturing temperature for 30 seconds completely inactivates the blocking antibodies, releasing both DNA polymerase and exonuclease activities. This dual blocking characteristic effectively prevents non-specific amplification caused by mismatches or primer dimers, and effectively inhibits the decrease in fluorescence signal due to probe degradation. This dual protection makes the in vitro detection reagent more stable during transportation or use at room temperature. Furthermore, this product undergoes an ultra-low residue processing process, resulting in extremely low levels of nuclease and host gDNA residues. Combined with an optimized buffer system, it effectively reduces non-specific amplification caused by primer-probe mixing during sample mixing, system heating, and long-term storage, supporting the development of pre-mixed primer-probe quantitative PCR systems. |
| Specification | 1000 U; 10000 U; 25000 U; 100000 U |
| Download Datasheet: |  |
