| Cat# | ENZD-RT17 |
| Specification | 200 rxns 1000 rxns 5000 rxns |
| Aplications | Diagnostic Pathogen Detection (Respiratory, Intestinal, etc.); Animal Pathogen Detection (African Swine Fever, etc.); Food Testing, etc. (Microbial, etc.) |
| Description | Suitable for rapid RT-qPCR detection of RNA templates, supporting single or multiple amplifications. Uses heat启动酶 and optimized buffer system, compatible with rapid protocols, and includes a built-in dUTP/UDG anti-contamination system. |
| Features | 1. Fully premixed stability: After full premixing of probes and primers, stable for 7 days at 37°C, 1 year at 4°C under pressure. 2. Rapid amplification: Stable detection within 22 minutes. 3. Direct amplification capability: Compatible with a large volume input of nasopharyngeal swab lysate at 1/2. |
| Quality Control | Fully premixed long-term stability 22 min fast amplification Compatible with 1/2 volume of lysate |
| Components | U+ One Step RT-qPCR Probe 5x Master Mix 50 × ROX Reference Dye 1 50 × ROX Reference Dye 2 |
| Download Datasheet: |  |
The ENZD-RT17 RT-qPCR premix system from Creative Enzymes was evaluated after full pre-mixing of primers and probes under both singleplex and multiplex conditions to assess long-term storage stability.
Compared with a -20°C control, the pre-mixed system maintained stable amplification performance after extended storage, showing up to 1 year stability under refrigerated conditions and 7 days under elevated stress conditions without loss of efficiency. The formulation integrates a dUTP/UDG contamination prevention system, supporting reliable performance during long-term storage and workflow handling. Data sourced from internal validation studies evaluating long-term stability of fully pre-mixed RT-qPCR systems.
ENZD-RT17 was tested across multiple singleplex and multiplex RT-qPCR systems using a rapid cycling protocol and compared with commercial one-step reagents under identical primer-probe conditions.
The results showed consistently lower CT values and faster signal generation across systems, enabling a 22-minute rapid amplification workflow with improved detection efficiency. The system also incorporates a dUTP/UDG contamination prevention system, ensuring reliable performance in fast cycling applications. Data sourced from internal validation studies evaluating rapid RT-qPCR amplification performance.
ENZD-RT17 was evaluated using crude lysates from nasopharyngeal swab samples prepared with different commercial lysis buffers. Input volumes of 5 μL, 7 μL, and 12.5 μL were tested in a 25 μL reaction system.
The premix maintained stable amplification across all tested input volumes and demonstrated full compatibility with up to 12.5 μL crude lysate, indicating strong tolerance to inhibitor-rich sample matrices. Data sourced from internal validation studies assessing crude sample compatibility in RT-qPCR workflows.
