| Cat# | ENZD-P05 |
| Specification | 500 U; 2500 U |
| Unit Concentration | 5 U/μl |
| Description | High-performance enzymes for ultramultiplex probe melting curves. EnzSmart U + Melt Pro Multiplex DNA Polymerase is a probe-based melting curve reagent specifically developed for TaqMan probe-based asymmetric PCR amplification technology. This product utilizes the EnzSmart platform to selectively screen enzymes with well-matched cleavage and polymerization activities, strong 3' mismatch recognition capabilities, and high tolerance to impurities. Combined with high-blocking-rate bispecific antibodies (binding to different antigenic epitopes) and an optimized buffer system, it significantly improves the melting curve peak, uniformity, specificity, GC content compatibility, and impurity tolerance (blood, guanidine salts, etc.) after ultra-multiplex amplification. Furthermore, the reagent incorporates a dUTP/UDG anti-contamination system to control residual contamination of amplified products, ensuring accurate results. It is a qPCR reagent suitable for ultra-multiplex probe melting curve platforms. |
| Features | 1. Superior amplification uniformity 2. Good specificity |
| Applications | Multiple pathogen detection; reproductive genetic testing; medication guidance; food testing, etc. |
| Components | EnzSmart U+ Melt Pro Multiplex DNA Polymerase (5 U/ul) 10 x PCR Buffer (Mg2+ plus) dNTP/dUTP Mix (10 mM each) MgCl2 (100 mM) |
| Shipping and Storage Conditions | Store at -30 ~ -15℃, transport at ≤0℃. |
| Download Datasheet: |  |
ENZD-P05 from Creative Enzymes was evaluated in an asymmetric multiplex PCR system (8-plex) alongside a comparable product from Supplier A. The results showed that ENZD-P05 generated higher melting peak signals across all detection channels, with improved amplification uniformity compared to the reference enzyme.
This performance highlights its capability as a high-performance hot-start enzyme with high blocking efficiency, ensuring balanced amplification in complex multiplex systems. Data generated from internal comparative performance studies.
In ultra-multiplex PCR systems, the probability of non-specific interactions between primers and probes increases significantly. ENZD-P05 was developed using a directed screening approach to achieve high blocking efficiency and high specificity as a hot-start enzyme, combined with an optimized buffer system.
Under conditions with complex primer–probe mixtures, ENZD-P05 maintained specific target amplification while effectively suppressing non-specific signals. Comparative testing with Supplier A using both target templates and no-template controls (NTC) demonstrated that ENZD-P05 significantly reduced the occurrence of non-specific peaks, confirming its superior specificity in high-complexity assays. Data generated from internal specificity evaluation studies.
