| Cat# | POL-024 |
| Notes | A brief incubation period is required during the 37°C analysis. |
| Storage | Store at -20 °C. |
| Activity | 10 U/μL |
| Description | T7 DNA polymerase is a template-dependent DNA polymerase that catalyzes DNA synthesis in the 5'→3' direction. This DNA polymerase exhibits high continuous synthesis capability, enabling the synthesis of long DNA fragments. The enzyme also demonstrates high 3'→5' exonuclease activity toward both single-stranded and double-stranded DNA. |
| Applications | Removal of residual genomic DNA and purification of covalently closed-circular DNA molecules; long-fragment primer extension reaction; 3'-end labeling of DNA; strand extension reaction at the target mutation site; 5'-overhang filling of DNA; second-strand cDNA synthesis; in situ detection of apoptosis-associated DNA fragments. |
| Specification | 300 U |
| Expression System | E.coli |
| 10× Reaction Buffer | 400 mM Tris-HCL (PH7.5 at 25°C), 100 mM MgCl₂, 10 mM DTT |
| Activity Definitions | The amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into a polynucleotide fragment (adsorbed onto DE-81) within 30 minutes at 37°C. |
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