| Cat# | ENZD-RT14 |
| Specification | 250 rxns（20 μl/rxn） |
| Aplications | Virus Detection; Pathogen Detection; Gene Expression Analysis |
| Description | Suitable for high-sensitivity quantitative detection of RNA templates, compatible with various RNA types, with stable and reliable amplification. Integrates reverse transcription and qPCR in one tube, supports multiple amplifications, and requires no complex optimization. |
| Features | 1. High detection sensitivity: Able to detect templates with a few copies or 0.1 pg total RNA. 2. Multiplex detection: Supports multiplex probe detection. |
| Components | RNase-free ddH2O 2 × One Step U+ Mix One Step U+ Enzyme Mix 50 × ROX Reference Dye 1 50 × ROX Reference Dye 2 |
| Shipping and Storage Conditions | Store at -20℃ in the dark |
| Download Datasheet: |  |
The ENZD-RT14 one-step RT-qPCR probe kit from Creative Enzymes was evaluated using multiple RNA template types to assess sensitivity, linear dynamic range, and multiplex performance. The system is powered by Taq DNA Polymerase, supporting stable amplification across diverse targets.
Using HeLa total RNA, amplification of the RPLPO gene demonstrated a linear range spanning five orders of magnitude with sensitivity down to 0.1 pg. With in vitro transcribed RNA, detection of the HCV target showed linearity across seven orders of magnitude with a limit of detection of 10 copies. In addition, viral RNA derived from serially diluted biological samples was reliably detected at high dilution levels, indicating strong performance in low-abundance conditions.
Multiplex evaluation using viral RNA further showed that dual-target amplification maintained comparable efficiency and sensitivity to singleplex reactions, confirming stable performance in multiplex assay formats. Data sourced from internal validation studies evaluating sensitivity, dynamic range, and multiplex amplification performance across diverse RNA templates.
