| Cat# | BDE-011 |
| Description | Purine nucleoside phosphorylase (PNP) is a key enzyme in the purine salvage pathway, conserved in prokaryotes and eukaryotes. It reversibly catalyzes the phosphorolysis of purine nucleosides to their corresponding base and ribose-1-phosphate, and is commonly used in assays for 5'-nucleotidase (5'-NT) and adenosine deaminase (ADA). |
| Applications | Used in enzymatic assays for purine metabolism-related indicators; it is often used in conjunction with xanthine oxidase. |
| CAS No. | 9030-21-1 |
| Enzyme Commission Number | EC 2.4.2.1 |
| Form | White amorphous powder, lyophilized |
| Source | Microorganism |
| Activity | ≥ 80 U/mg-solid; ≥ 190 U/mg-protein |
| Unit Definition | One unit causes the formation of one micromole of uric acid per minute at pH 7.7 at 37°C. |
| Molecular Weight | 35 kDa (SDS-PAGE) |
| Optimum pH | 8.0-8.5 |
| Optimum temperature | 55°C |
| Storage | Aliquot and store at ≤ -20 °C. |
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