| Cat# | ENZD-RT16 |
| Specification | 250 rxns（20 μl/rxn） |
| Description | Uses the SYBR Green I dye method, completing RT and qPCR in one step, simple to operate and reduces the risk of contamination. Has high sensitivity (up to 1 pg total RNA), and the system is optimized to reduce primer dimer formation. |
| Features | 1. Excellent specificity: Special additives significantly inhibit the formation of primer dimers, resulting in more accurate quantitative results. 2. High detection sensitivity: Combining high amplification efficiency, can detect as low as 1 pg of total RNA template. |
| Components | RNase-free ddH2O 2 × One Step U+ Mix One Step U+ Enzyme Mix 50 × ROX Reference Dye 1 50 × ROX Reference Dye 2 |
| Shipping and Storage Conditions | Store at -20℃ in the dark |
| Download Datasheet: |  |
The ENZD-RT16 one-step RT-qPCR SYBR Green kit from Creative Enzymes was assessed using HeLa total RNA for amplification of the B2M and GUSB genes, in comparison with commercially available SYBR Green reagents.
ENZD-RT16 showed improved amplification efficiency, reflected by lower CT values across both targets. Notably, minimal primer-dimer formation was observed even at low template input levels, indicating high specificity and clean amplification profiles.
These results demonstrate reliable performance in gene expression analysis, with strong signal clarity and reduced background interference. Data sourced from internal validation studies evaluating SYBR Green-based RT-qPCR performance and primer-dimer formation.
