| Cat# | ENZD-RT11 |
| Specification | 100 rxns 1000 rxns 5000 rxns |
| Aplications | Virus Detection; Pathogen Detection; Fusion Gene Detection; DNA/RNA Co-Detection; Gene Expression Analysis; Drug Residue Detection |
| Description | Suitable for quantitative detection of RNA templates (such as RNA viruses), integrating reverse transcription and qPCR in one tube to improve efficiency and reduce contamination risk. Includes a built-in dUTP/UDG anti-contamination system, compatible with TaqMan and other probe-based detection methods, and offers an optional glycerol-free version. |
| Features | 1. Higher detection sensitivity. 2. Suitable for multiplex qPCR. 3. Compatible with fast protocols. 4. Superior storage stability. 5. Balanced amplification of high and low template concentrations. 6. Introduction of dUTP/UDG anti-contamination system. |
| Quality Control | Excellent amplification performance Superior storage stability |
| Components | RNase-free ddH2O 5 × One Step U+ Mix One Step U+ Enzyme Mix 50 × ROX Reference Dye 1 50 × ROX Reference Dye 2 |
| Shipping and Storage Conditions | Store at -30 ~ -15℃, transport at ≤0℃. |
| Download Datasheet: |  |
The ENZD-RT11 one-step qRT-PCR probe kit from Creative Enzymes was evaluated in both singleplex and multiplex amplification systems and compared with leading commercial reagents under identical reaction conditions.
Across tested systems, ENZD-RT11 demonstrated superior amplification performance, with consistently stronger signal output and improved amplification efficiency in both single-target and multi-target detection formats. The system is built on a hot-start Champagne Taq DNA Polymerase platform, ensuring precise reaction initiation and high amplification specificity. In addition, the integrated dUTP/UDG contamination prevention system effectively reduces carryover risk, supporting reliable quantitative performance. Data sourced from internal validation studies evaluating qRT-PCR amplification efficiency in singleplex and multiplex systems.
ENZD-RT11 was subjected to stability assessment under multiple stress conditions, including repeated freeze–thaw cycles (10, 30, and 50 cycles) and accelerated incubation at 37°C for 1, 3, 5, and 7 days. Performance was compared with a standard -20°C storage control across different template concentrations and assay systems.
The results showed that ENZD-RT11 maintained consistent amplification performance across all stress conditions, with negligible variation compared to the control group. Both signal intensity and detection efficiency remained stable, demonstrating strong resistance to thermal and mechanical stress. Data sourced from internal validation studies evaluating freeze–thaw stability and accelerated thermal storage performance.
