| Cat# | ENZD-RT19 |
| Specification | 200 rxns (20 μl/rxn) 1000 rxns (20 μl/rxn) 10000 rxns (20 μl/rxn) |
| Aplications | Molecular POCT Platform; Pathogen Detection; Fusion Gene Detection; DNA/RNA Co-Detection; Gene Expression Analysis |
| Description | Single-tube probe-based RT-qPCR premix, suitable for single or multiple detection of RNA templates. Uses hot start DNA Polymerase and optimized buffer system, supports premixed primers and probes and rapid protocols, and includes a built-in dUTP/UDG anti-contamination system, easy to operate. |
| Features | 1. Probe primers pre-mixed: Probe primers can be added to the premix in advance, maintaining stable performance. 2. DNA/RNA co-detection: Stably achieves sensitive amplification of DNA and RNA templates. 3. Sensitive and efficient: Stable detection of single-digit copy templates. 4. Multiple amplification: Compatible with 4-5 fold amplification. |
| Quality Control | Supports fully premixed reagents with probe and primer pre-added Template flexibility |
| Components | RNase-free ddH2O U+ One Step RT-qPCR Probe 5x Master Mix 50 × ROX Reference Dye 1 50 × ROX Reference Dye 2 |
| Shipping and Storage Conditions | Store at -30 ~ -15℃, transport at ≤0℃. |
| Download Datasheet: |  |
To evaluate workflow convenience, the ENZD-RT19 one-step RT-qPCR super premix system from Creative Enzymes was tested in a format where primers and probes were fully incorporated into a ready-to-use reaction mixture in advance.
The pre-assembled systems were subjected to different storage and stress conditions, including refrigerated storage for 14 days, elevated stress for 5 days, and 50 freeze–thaw cycles, followed by performance comparison against freshly prepared reactions. No noticeable loss in amplification efficiency was observed across all conditions, indicating strong formulation stability. The system is built on a next-generation Taq DNA Polymerase with a warm-start reverse transcriptase design, together with a dUTP/UDG contamination control system, enabling reliable performance even in fully pre-mixed formats where only template addition is required at the time of use. Data sourced from internal validation studies evaluating stability of pre-assembled RT-qPCR reaction systems under storage and stress conditions.
The amplification behavior of ENZD-RT19 was further examined in multiplex reactions containing both RNA and DNA templates within the same assay system.
Both target types were successfully detected in a single reaction setup, maintaining consistent amplification efficiency and specificity across mixed-template conditions. This indicates that the system is well-suited for applications requiring simultaneous detection of different nucleic acid types. The formulation integrates a next-generation Taq DNA Polymerase, a warm-start reverse transcriptase for controlled initiation, and a dUTP/UDG contamination prevention system, ensuring stable performance in complex multiplex environments. Data sourced from internal validation studies evaluating multiplex performance in mixed RNA/DNA detection systems.
