| Cat# | ENZD-QM09 |
| Specification | 500 rxns（20 μl/rxn） 2500 rxns（20 μl/rxn） |
| Aplications | Gene Expression Analysis; High Sensitivity qPCR Reaction |
| Description | Designed specifically for high specificity and high sensitivity real-time quantitative PCR, containing AceTaq HS DNA Polymerase and optimized buffer system, effectively suppressing non-specific amplification. Suitable for various probe types including TaqMan, MGB, and Molecular Beacon, 2× premix form, compatible with mainstream qPCR instruments, and offers optional ROX reference dye at different concentrations. |
| Features | 1. Excellent amplification sensitivity: Based on hot-start AceTaq DNA Polymerase, providing excellent amplification performance and sensitivity 2. Robust repeatability: Reliable quantitative results, meeting the data requirements of high-level journals |
| Components | 2 × qPCR Probe Master Mix\n50 × ROX Reference Dye 1\n50 × ROX Reference Dye 2 |
| Shipping and Storage Conditions | Store at -30 ~ -15℃. |
| Download Datasheet: |  |
The analytical performance of ENZD-QM09 was assessed using a tenfold serial dilution of pUC19 plasmid DNA prepared in HeLa cell cDNA background. The final dilution point corresponded to approximately 8 copies per 4 μL reaction, with 4 μL template input per well.
Across the full dilution series, ENZD-QM09, a qPCR probe master mix developed by Creative Enzymes, exhibited strong linear amplification behavior with high quantitative accuracy. The assay achieved 98% amplification efficiency with an R2 value of 0.9991, demonstrating excellent detection consistency and reliable quantification down to single-digit copy levels. These results highlight the excellent storage stability and strong performance robustness of the formulation in low-copy-number detection systems. Data sourced from internal validation studies evaluating amplification efficiency, linearity, and low-copy sensitivity.
