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Universal U+ Probe Master Mix V2

Cat# ENZD-QM03
Specification 500 rxns(20 μl/rxn)
2500 rxns(20 μl/rxn)
Aplications Gene Expression Analysis
Description Adopting chemically modified enzyme and optimized buffer system, the sensitivity and amplification efficiency of probe-based qPCR are enhanced. Equipped with dUTP/UDG anti-contamination system and ROX passive reference dye, it is compatible with various qPCR instruments and easy to use.
Features 1. Outstanding amplification sensitivity: The rigorous chemical method hot-start enzyme Acetaq DNA Polymerase, combined with a carefully optimized buffer system, can detect down to single-copy templates.
2. Excellent linearity: Exhibits good linearity within a wide template range, with an amplification efficiency of up to 99%.
3. dUTP/UDG anti-contamination system: The introduction of Heat-Labile UDG anti-contamination system rapidly degrades U-containing contaminants at room temperature, eliminating the impact of amplification product contamination on qPCR reactions.
4. Wide platform compatibility: Special ROX reference dye suitable for all qPCR instruments, no need to adjust ROX concentration on different qPCR instruments.
Components 2 × Universal U+ Probe Master Mix V2(20 µl/run), including dNTP/dUTP Mix, Mg²⁺, AceTaq HS DNA Polymerase, Heat-labile UDG, Specific ROX Reference Dye, etc.
Shipping and Storage Conditions Store at -20℃ in the dark.

Store at 4℃ for 3 weeks; can be frozen and thawed 30 times;

The prepared mixture (primers, template, reagents) can be stored at 4℃ for 6 hours.
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For research and industrial use only, not for personal medicinal use.

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