| Cat# | ENZD-RT10 |
| Specification | 200 rxns 1000 rxns 5000 rxns |
| Aplications | Diagnostic Pathogen Detection (Respiratory, Intestinal, etc.); Animal Pathogen Detection (African Swine Fever, etc.); Food Testing, etc. (Microbial, etc.) |
| Description | Suitable for rapid RT-qPCR detection of RNA templates, with fast amplification speed and high efficiency. Contains a dUTP/UDG anti-contamination system, supports multiple amplifications, and ensures detection accuracy. |
| Features | 1. High sensitivity: Superior to common products on the market, detectable about 1 CT value earlier. 2. Rapid amplification: Stable detection within 22 minutes. 3. Direct amplification capability: Compatible with a large volume input of nasopharyngeal swab lysis solution 1/2. |
| Quality Control | High efficiency and sensitivity 22 min fast amplification Compatible with 1/2 volume of lysate |
| Components | 5 × One Step U* Mix One Step U* Enzyme Mix 50 × ROX Reference Dye 1 50 × ROX Reference Dye 2 |
| Shipping and Storage Conditions | Store at -30 ~ -15℃, transport at ≤0℃. |
| Download Datasheet: |  |
The ENZD-RT10 multiplex qPCR premix from Creative Enzymes was evaluated in both singleplex and multiplex assay systems and compared with commonly used commercial reagents.
Across most tested systems, ENZD-RT10 exhibited 1–2 CT lower signals, indicating higher sensitivity and improved amplification efficiency. The system also showed well-balanced signal output in multiplex reactions, supporting reliable multi-target detection. In addition, the premix integrates a dUTP/UDG contamination prevention system, effectively minimizing carryover contamination and improving assay reliability.
Data sourced from internal validation studies evaluating multiplex amplification performance and contamination control efficiency.
ENZD-RT10 was tested across multiple singleplex and multiplex systems using a rapid amplification workflow under identical primer-probe conditions and compared with a commercial one-step reagent.
The results demonstrated that ENZD-RT10 achieved faster detection with lower CT values, supporting a rapid 22-minute amplification performance while maintaining stable signal output across different assay systems.
The system also retained consistent performance under multiplex conditions, confirming strong suitability for fast diagnostic workflows. Data sourced from internal validation studies evaluating rapid amplification performance across multiple qPCR systems.
ENZD-RT10 was evaluated using crude lysates derived from nasopharyngeal swab samples prepared with different commercial lysis buffers. Various input volumes (5 μL, 7 μL, and 12.5 μL) were tested in a 25 μL reaction system.
The results showed that ENZD-RT10 maintained stable amplification performance across different input volumes and was fully compatible with up to 12.5 μL crude lysate, demonstrating strong tolerance to inhibitor-rich sample backgrounds. Data sourced from internal validation studies assessing crude sample compatibility under varying lysis input conditions.
