| Cat# | ENZD-RT23 |
| Specification | 50 rxns |
| Aplications | Virus Detection; Gene Expression Analysis; Endpoint PCR Detection |
| Description | One-step RT-PCR kit, uses optimized enzyme combination and buffer system to improve specificity and sensitivity. Contains gDNA Wiper to reduce genomic contamination, can detect as low as 0.1 pg total RNA and stably amplify up to 10 kb fragments. |
| Features | 1. High yield: The gel band is deep and bright. 2. High sensitivity: Can detect as little as 0.1 pg of RNA sample. 3. gDNA clearance: Provides a gDNA clearance module to avoid genomic DNA residues. |
| Quality Control | High yield High sensitivity |
| Components | 5 x gDNA Wiper Mix 2x One Step RT-qPCR Master Mix（Dye Plus） |
| Shipping and Storage Conditions | Store at -30 ~ -15℃, transport at ≤0℃. |
| Download Datasheet: |  |
The RT-PCR systems from Creative Enzymes (ENZD-RT23 and ENZD-RT24) were evaluated for amplification yield using long RNA targets and compared against commonly used commercial one-step RT-PCR kits (Supplier 1–5).
Across target fragments of 1289 bp, 3192 bp, and 5163 bp, both ENZD-RT23 and ENZD-RT24 consistently produced higher amplification output under identical template input conditions. This indicates improved extension efficiency and stronger performance in long-fragment transcription–amplification workflows compared with reference systems. Data sourced from internal validation studies evaluating RT-PCR yield across long-fragment RNA targets.
ENZD-RT23 and ENZD-RT24 were further assessed for sensitivity using low-concentration RNA templates (0.1 pg and 1 pg, ~1 kb target) and benchmarked against commercial RT-PCR reagents.
Both systems achieved reliable detection down to 0.1 pg input, while maintaining higher amplification output compared with Supplier 1–5 across tested conditions. The results demonstrate strong sensitivity combined with efficient signal generation at very low template abundance. The system demonstrates reliable performance in low-copy RNA detection while preserving strong product yield. Data sourced from internal validation studies evaluating RT-PCR sensitivity under low RNA input conditions.
