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Diaphorase from Microorganism

Cat# DIA-423
Source Microorganism
Activity ≥ 600.0 U/mg
Isoelectric point 5.2
Storage −25 °C to −15 °C
Enzyme Commission Number EC 1.6.5.2
Purity ≥ 90.0% (SDS-PAGE)
pH Stability 4.5–10.0
Michaelis Constant 7.4 × 10-4 M (NADH)
6.0 × 10-2 M (NADPH)
Optimum pH 7.5
Optimum temperature 45 °C
Thermal stability Below 50 °C (pH 7.5, 30 min)
Inhibitors Fe3+, Mn2+, Cu2+
Applications For colorimetric determination of NAD(P)H and many dehydrogenases when coupled with various dyes which act as hydrogen acceptors from NAD(P)H.
Molecular Weight 28 kDa (SDS-PAGE)
Package 5/100/500/1000 KU
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