Sarcosine Oxidase from E. coli, Recombinant
| Cat# | DIA-414 |
| Abbr | SAO, Recombinant (E. coli) |
| Alias | SAO |
| Species | E. coli |
| Source | E. coli |
| Description | Sarcosine oxidase (SAO) is an enzyme that catalyzes the oxidative demethylation of sarcosine to yield glycine, H2O2, 5, 10-CH2-tetrahydrofolate in a reaction requiring H4-tetrahydrofolate and oxygen. sarcosine + H2O + O2 = glycine + formaldehyde + H2O2. |
| Appearance | Yellow lyophilizate |
| Enzyme Commission Number | EC 1.5.3.1 |
| Activity | > 10 U/mg |
| Contaminants | catalase < 0.5% glucose oxidase < 1.0 x 10^-5% |
| Molecular Weight | ca. 49 kDa |
| Isoelectric point | 5.3 |
| pH Stability | 6.5–10.5 |
| Michaelis Constant | 4.7 x 10^-3 M (sarcosine) |
| Structure | monomer of 43 kDa (SDS-PAGE) one mole of FAD per mole of enzyme |
| Unit Definition | One unit (U) is defined as the amount of enzyme which produces 1 μmol of hydrogen peroxide per min at 37°C and pH 7.7. |
| Optimum pH | 6.7–9.5 |
| Optimum temperature | 50°C |
| Thermal stability | below 55°C |
| Stability | Stability (liquid form) stable at 37°C for at least two weeks Stability (powder form) stable at 30°C for at least one month |
| Storage | at -20°C |
| Stabilizers | Sucrose |
| Inhibitors | Zn2+, Cu2+, Hg2+, Ag+ |
| Synonyms | Sarcosine Oxidase; EC 1.5.3.1; SAO |
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