| Cat# | NATE-1242 |
| Abbr | Creatininase, Recombinant (E. coli) |
| Species | E. coli |
| Source | E. coli |
| Description | Creatininase from Pseudomonas sp. is a homohexameric enzyme with a molecular mass of 28.4 kDa per subunit. It is a cyclic amidohydrolase catalysing the reversible conversion of creatinine to creatine. Each monomer contains a binuclear zinc centre near the C termini of the β-strands and the N termini of the main α-helices. These zinc ions indicate the location of the active site. |
| Appearance | White lyophilizate |
| Enzyme Commission Number | EC 3.5.2.10 |
| Activity | > 100 U/mg |
| Contaminants | catalase < 1.0% |
| Molecular Weight | ca. 170 kDa |
| Isoelectric point | 4.8 |
| pH Stability | 7.0–11.0 |
| Michaelis Constant | 3.4 x 10^-2 M (creatinine) 4.3 x 10^-2 M (creatine) |
| Structure | 6 subunits of 28 kDa (SDS-PAGE) |
| Unit Definition | One unit (U) is defined as the amount of enzyme which produces 1 μmol of creatine per min at 37°C and pH 6.8. |
| Optimum pH | 6.5–7.0 |
| Optimum temperature | 60–65°C |
| Activators | Mg2+, Mn2+ |
| Thermal stability | below 60°C |
| Storage | at -20°C |
| Inhibitors | Hg2+ |
| Synonyms | EC 3.5.2.10, creatinine hydrolase; Creatininase; 9025-13-2 |
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