| Cat# | DIA-583 |
| Source | Escherichia coli |
| Description | This N-terminally truncated form of DNA polymerase I retains DNA polymerase activity but lacks 5′→3′ exonuclease activity. Additional mutations (D355A, E357A) eliminate its 3′→5′ exonuclease activity. As a result, the enzyme provides polymerase activity without proofreading, making it suitable for applications requiring controlled nucleotide incorporation. |
| Unit Definition | One unit is defined as the amount of enzyme required to incorporate 10 nmol of dNTP into acid-insoluble material within 30 minutes at 37 °C. |
| Storage | -20 °C |
| Buffer | 25 mM Tris-HCl pH 7.4, 0.1 mM EDTA, 1 mM DTT, 50% glycerol |
| Applications | Dideoxy sequencing; random primer labeling; 3′ dA-tailing during library preparation. |
| Product Overview | 3′ dA-tailing. Probe preparation using random primers. Random primer labeling. |
| Package | 5000 U Klenow Fragment exo- (5 U/μL): 1 mL 10× Blue Buffer (100 mM Tris-HCl pH 7.9, 500 mM NaCl, 100 mM MgCl2, 10 mM DTT): 2 mL |
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