| Cat# | POL-001 |
| MW | 70 kDa (Reducing) |
| Form | Liquid |
| Label | His Tag |
| Notes | Due to the 3′→5′ exonuclease activity of the enzyme, increasing the reaction temperature, adding too much enzyme, not adding dNTP or too long reaction time will lead to the formation of the dented end. Please avoid repeated freeze-thaw cycles |
| Purity | ＞95% by SDS-PAGE and HPLC |
| Storage | Store at -25 ~ -15°C for 2 years. |
| Activity | 5 U/μL |
| Synonyms | Klenow Fragment DNA Polymerase I Large (Klenow) Fragment |
| Component | 5 U/μL Klenow Fragment, 25 mM Tris-HCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.4 at 25°C |
| Description | The Klenow Fragment, is a large fragment of E.coli. DNA polymerase I. It retains the 3′→5′ exonuclease activity of DNA polymerase I, but lacks the 5′→3′ exonuclease activity of the intact DNA polymerase I. |
| Applications | The 3′→5′ exonuclease activity of Klenow Fragment ensures accurate proofreading when synthesizing DNA. It is used to fill in the 5′ overhang ends of double-stranded DNA; and double-stranded DNA 3′ overhang flattening (also called trimming). It can also be used for the synthesis of the second strand of cDNA or the synthesis of the second strand of site-specific mutation reaction. |
| Specification | 200 U; 1000 U |
| Dilution Buffer | 25 mM Tris-HCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.4 at 25°C |
| Molecular Marker | Unconjugated |
| Expression System | E.coli |
| 10× Reaction Buffer | 50 mM NaCl, 10 mM Tris-HCl, 10 mM MgCl₂, 1 mM DTT (pH 7.9 at 25°C). |
| Activity Definitions | One unit is defined as the amount of enzyme required to incorporate 10 nmol of dNTP into acid insoluble substances at 37 °C for 30 minutes. |
| Download Datasheet: |  |
