| Cat# | BDE-047 |
| Description | Homocysteine (Hcy) is a sulfur-containing amino acid and an established independent risk factor for cardiovascular disease. The enzymatic cycling method for Hcy detection is rapid, sensitive, and compatible with automated biochemical analyzers; homocysteine methyltransferase (HMT) is one of its core enzymes. |
| Applications | Used in homocysteine assay kits, participating in the enzymatic coupling reaction of the methionine cycle. |
| Form | Colorless clear liquid or lyophilized powder |
| Source | Recombinant protein expression |
| Activity | 70 U/mg |
| Unit Definition | One unit is defined as the amount of enzyme required to catalyze a reaction that causes a decrease of 0.01 in the absorbance of NADH at 340 nm per minute under specified assay conditions (37 °C, pH 8.3). |
| Molecular Weight | 51 kDa (SDS-PAGE) |
| Purity | ≥ 90% by SDS-PAGE |
| Optimum pH | 6.5-8.5 |
| Isoelectric Point | 5.01 |
| Storage | Store at -20°C for at least one year, or at -80°C for two years. |
| Notes | It can be resuspended in purified water containing 30% glycerol and stored at 4°C for approximately one year; After aliquoting according to usage, store at -20°C for up to two years; avoid repeated freeze-thaw cycles as much as possible. |
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