Uricase from E. coli, Recombinant
| Cat# | DIA-415 |
| Source | E. coli |
| Description | The enzyme urate oxidase (UO), or uricase or factor-independent urate hydroxylase, absent in humans, catalyzes the oxidation of uric acid to 5-hydroxyisourate: Uric acid + O2 + H2O → 5-hydroxyisourate + H2O2 → allantoin + CO2 |
| Activity | > 4 U/mg lyophilizate |
| CAS No. | 9002-12-4 |
| Unit Definition | One unit (U) is defined as the amount of enzyme which oxidizes 1 μmol of uric acid per min at 25°C and pH 8.5. |
| Storage | at -20°C |
| Synonyms | urate oxidase; uric acid oxidase; uricase; uricase; urate: oxygen oxidoreductase; EC 1.7.3.3; uricase II |
| Enzyme Commission Number | EC 1.7.3.3 |
| pH Stability | 7.0–11.0 |
| Michaelis Constant | 1.1 x 10^-5 M (uric acid) |
| Optimum pH | 8.5 |
| Optimum temperature | 45°C |
| Thermal stability | below 55°C |
| Stability | Stability (liquid form) stable at 37°C for at least ten days Stability (powder form) stable at 30°C at least three weeks |
| Stabilizers | Citrate, sucrose |
| Inhibitors | Hg2+, Ag+ |
| Contaminants | catalase < 1.0% |
| Abbr | UO, Recombinant (E. coli) |
| Alias | UO |
| Appearance | Light brownish lyophilizate |
| Structure | 2 subunits of 35 kDa (SDS-PAGE) |
| Molecular Weight | ca. 90 kDa |
| Species | E. coli |
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