| Cat# | ENZD-QM08 |
| Specification | 500 rxns（20 μl/rxn） |
| Aplications | SNP Genotyping |
| Description | Used for TaqMan probe-based SNP typing, equipped with high-performance Champagne Taq DNA Polymerase, improving typing accuracy and amplification success rate. It contains Heat-labile dUTP/UDG and ROX reference dye, compatible with various qPCR instruments, and is easy to operate. |
| Features | 1.Flexible template input: compatible with 1-10 ng of genomic input, and still performs excellently in typing results with low template input. 2. Broad GC compatibility: accurate typing at SNP sites with GC content ranging from 25% to 73%. 3. Excellent storage stability: Fluorescence signals are stable after PCR mix preparation for 72 hours at room temperature in the dark; 4. Fluorescence signals are stable after PCR reaction for 72 hours and endpoint signal reading. Typing results are good both pre-PCR and post-PCR for 72 hours. 5. Compatible with direct amplification from blood lysate: No need to extract genomic DNA from blood, SNP typing can be performed using blood lysate as the template, with typing results comparable to those using genomic DNA as the template. |
| Quality Control | 1. Flexible template input 2. Wide GC compatibility 3. Excellent storage stability 4. Compatible with direct amplification from blood lysate |
| Components | 2 × Hot-start Geno-SNP Probe Master Mix(20 µl/run), including dNTP/dUTP Mix, Mg²⁺, Champagne Taq DNA Polymerase, Heat-labile UDG, Specific ROX Reference Dye, etc. |
| Shipping and Storage Conditions | Store at -30℃ -15℃ in the dark. Store at 4℃ for 3 weeks; store at 37℃ for 7 days; can be frozen and thawed 30 times; The prepared mixture (primers, template, reagents) can be stored at 4℃ for 72 hours. |
| Download Datasheet: |  |
The performance of ENZD-QM08 was evaluated using human genomic DNA samples at different template input levels for SNP genotyping at the rs4947963 locus.
Across 50 human gDNA samples, robust genotyping results were obtained using both 10 ng and 1 ng DNA per reaction. Even at reduced template input (1 ng/rxn), ENZD-QM08 maintained clear and consistent allelic discrimination, demonstrating strong adaptability in low-input SNP analysis workflows. This system is developed by Creative Enzymes and optimized for high-efficiency genotyping performance. Data sourced from internal validation studies evaluating SNP genotyping performance at varying template input levels.
ENZD-QM08 was tested across a panel of 80 human genomic DNA samples targeting two SNP loci with distinct GC compositions: rs951134 (25% GC) and rs12214 (73% GC).
Results showed consistent and accurate genotype clustering at both low-GC and high-GC loci, indicating that ENZD-QM08 maintains stable amplification behavior across a wide GC range and minimizes GC-related amplification bias in SNP assays. Data sourced from internal validation studies evaluating GC-dependent performance in SNP genotyping assays.
ENZD-QM08 was evaluated for storage robustness under extended room-temperature exposure. After PCR setup, reaction mixtures were kept at room temperature in the dark for 72 hours prior to amplification. In addition, post-PCR products were also stored under the same conditions for 72 hours before signal acquisition.
Across both pre-PCR and post-PCR conditions, genotyping results remained consistent with untreated controls, indicating strong stability during extended handling periods. This confirms the excellent storage stability of ENZD-QM08, enabling flexible workflow scheduling in high-throughput SNP analysis. Data sourced from internal validation studies evaluating pre-PCR and post-PCR room-temperature stability.
The compatibility of ENZD-QM08 with crude biological samples was assessed using blood-derived lysate without prior genomic DNA extraction.
Genotyping results at the rs4947963 locus showed comparable performance between blood lysate templates and purified genomic DNA templates, indicating that ENZD-QM08 supports direct SNP genotyping from blood-derived lysates with maintained accuracy and reliability. Data sourced from internal validation studies evaluating direct genotyping performance using blood lysate and genomic DNA templates.
