Endo-Beta-N-acetylglucosaminidase M (EndoM) from Mucor hiemalis hydrolyzes both high-mannose and complex type N-linked glycans, releases intact oligosaccharide chains while leaving one GlcNAc residue on the polypeptide, and supports versatile glycoprotein deglycosylation and glycan analysis.
Applications
Endo-Beta-N-acetylglucosaminidase M (EndoM) serves as a tool for versatile N-glycan detection kits and glycoprotein deglycosylation assay reagent development.
CAS No.
32-1-961
Enzyme Commission Number
EC 3.2.1.96
Enzyme Source
E.coli recombinant Endo M from Mucor hiemalis.
Activity Definition
One unit is defined as the amount of enzyme required to remove > 95% of the carbohydrate from 10 Mug of denatured RNase B in 1 hour at 37°C in a total reaction volume of 10 Mul.