| Cat# | POL-021 |
| Storage | Store at -20 °C. |
| Features | Two buffers are provided: one containing Mg²⁺ and another without Mg²⁺. Select the appropriate buffer based on the experimental purpose. |
| Component | 20 mM Tris-HCl (pH 8.0), 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.5% Nonidet P-40, 0.5% Tween 20, 50% Glycerol |
| Description | This enzyme is a recombinant form produced by expressing the thermostable Taq DNA polymerase gene, cloned from Thermus aquaticus YT-1, in Escherichia coli. It possesses the same properties as native Taq DNA polymerase. Due to its high purity, it exhibits exceptional specificity. This product is a 2× Master Mix containing Taq DNA Polymerase (enabling hot start PCR and pre-loaded with electrophoresis dye). Simply add template DNA and primers to perform PCR; reaction products can be directly analyzed by electrophoresis. The pre-incorporated Taq antibody in the Master Mix enables highly specific and efficient amplification through the hot start mechanism. |
| Applications | PCR |
| Specification | 250 U |
| Expression System | E.coli |
| 10× Reaction Buffer | 100 mM Tris-HCl (pH 8.3), 500 mM KCl, 15 mM MgCl₂ |
| Activity Definitions | Under the activity measurement conditions at 75°C, the enzyme activity required to convert 10 nmoles of total nucleotides into acid-insoluble products within 30 minutes is defined as 1 U. |
| Download Datasheet: |  |
