| Cat# | ENZD-MDLyo04 |
| Specification | 1000 U; 5000 U |
| Unit Concentration | 5 U/μl |
| Description | This product is a hot-start Taq polymerase obtained by mixing Champagne Taq antibody and Taq DNA polymerase in a specific ratio. Based on the thermostability of Champagne Taq antibody, Taq HS DNA polymerase maintains strict blocking properties at 55°C, minimizing non-specific amplification during sample mixing and temperature rise. When the reaction is held at 95°C for more than 30 seconds, Champagne Taq antibody is inactivated, and Taq enzyme activity is fully released, ensuring high amplification sensitivity and specificity in the PCR system. Activation of Taq HS DNA polymerase is unaffected by buffer pH, ionic strength, or other factors, making it suitable for various hot-start PCR and qPCR reactions based on Taq DNA polymerase. It is commonly used to amplify low-copy genes from complex templates (genomic DNA, cDNA) and is a hot-start Taq enzyme based on PCR/qPCR molecular diagnostic reagents. This product is a glycerol-free Taq HS DNA polymerase and can be lyophilized. |
| Notes | The amplification is highly sensitive and specific, ensuring reliable results. |
| Features | Heating at 95℃ for 30 seconds will completely release Taq enzyme activity. High amplification sensitivity and specificity. Compatible with various PCR/qPCR systems. |
| Applications | Gene expression analysis of low-copy genes in complex templates. |
| Components | Taq HS DNA polymerase (Glycerol-free) (5 U/µl) |
| Quality Control | Excellent amplification performance; Broad system compatibility |
| Shipping and Storage Conditions | Store at -30 ~ -15℃, transport at ≤0℃. |
| Download Datasheet: |  |
The amplification performance of ENZD-MDLyo04 was evaluated by qPCR in comparison with three commercial reference products under identical reaction conditions using the same primer–probe sets.
The results demonstrated improved amplification performance, reflected by higher sensitivity and more efficient signal generation. In addition, ENZD-MDLyo04 exhibited a higher and more stable fluorescence plateau phase, indicating sustained amplification efficiency and robust signal output during the later cycles.
As a hot-start Taq DNA polymerase-based system, ENZD-MDLyo04 effectively reduces non-specific amplification while maintaining strong reaction specificity and amplification efficiency, supporting consistent and reliable detection performance. Data generated from internal validation studies, demonstrating enhanced amplification sensitivity and consistent signal output.
To assess system compatibility, ENZD-MDLyo04 was evaluated in multiplex qPCR assays across three fluorescence channels (FAM, VIC, and CY5) and compared with multiple commercial reference reagents.
Under identical experimental conditions, ENZD-MDLyo04 demonstrated comparable or superior performance to leading reference products, maintaining stable amplification signals across all detection channels. The observed performance ranking (ENZD-MDLyo04 ≈ Supplier M > Supplier T > Supplier P) highlights its strong compatibility with complex multiplex systems.
These results confirm that ENZD-MDLyo04 provides excellent amplification performance and broad system compatibility, making it well-suited for multi-target detection workflows. Data generated from internal validation studies, confirming consistent performance across multiplex qPCR detection channels.
