Endo-Beta-N-acetylglucosaminidase (Double antenna complex type) (Endo S) from Streptococcus pyogenes specifically cleaves biantennary complex N-glycans on IgG antibodies, shows high specificity for the Fc glycosylation site, and is the gold standard tool for antibody glycan analysis and glycoengineering.
Applications
Endo-Beta-N-acetylglucosaminidase (Double antenna complex type) (Endo S) is widely used in antibody Fc glycosylation detection kits and IgG glycan profiling assay reagent development.
CAS No.
32-1-96
Enzyme Commission Number
EC 3.2.1.96
Enzyme Source
Streptococcus pyogenes
Activity Definition
One unit is defined as the amount of enzyme required to remove > 95% of the carbohydrate from 5 Mug of native mouse monoclonal IgG in 1 hour at 37°C in a total reaction volume of 10 Mul