| Cat# | ENZD-RT18 |
| Specification | 250 rxns |
| Aplications | Gene Quantification; Gene Expression Analysis; Virus Detection |
| Description | One-step SYBR Green RT-qPCR reagent with high sensitivity and specificity. Contains a UDG anti-contamination system and gDNA Wiper Mix to reduce genomic contamination, and comes with a universal ROX reference dye, compatible with mainstream qPCR instruments. |
| Features | 1. Excellent performance: High sensitivity and specificity, compatible with more systems for amplification. 2. Anti-contamination: Contains dUTP/UDG anti-contamination system, effectively preventing contamination of amplification products. 3. gDNA clearance: Provides a gDNA clearance module to avoid genomic DNA residues. |
| Quality Control | High sensitivity and specificity UDG anti-contamination system added |
| Components | 5x gDNA Wiper Mix 2 x One step SYBR Green Master Mix |
| Shipping and Storage Conditions | Store at -30 ~ -15℃, transport at ≤0℃. 2 × One Step SYBR Green Master Mix store at -30 ~ -15℃ in the dark. |
| Download Datasheet: |  |
The ENZD-RT18 RT-qPCR system from Creative Enzymes was evaluated using templates with varying GC content alongside a non-specific amplification setup to assess overall assay sensitivity and specificity.
ENZD-RT18 consistently delivered higher sensitivity and improved amplification efficiency across GC-variable targets compared with the reference reagent. In non-specific reactions, a single sharp peak was observed in melt curve analysis, indicating strong specificity and minimal background amplification. The system is formulated with a next-generation Taq DNA Polymerase and a dedicated warm-start reverse transcriptase, enabling precise reaction control and high-fidelity amplification. Data sourced from internal validation studies evaluating amplification sensitivity and specificity across GC-variable and non-specific systems.
The contamination control efficiency of ENZD-RT18 was assessed using fully uracil-containing and thymine-containing templates at 4 pg, 40 pg, and 400 pg input levels.
Results showed consistently high removal efficiency of carryover templates, with calculated performance exceeding 99% across all tested concentrations. This is achieved through an integrated dUTP/UDG contamination prevention system, ensuring effective elimination of uracil-based contamination while maintaining robust amplification performance. Data sourced from internal validation studies evaluating dUTP/UDG-based contamination control efficiency.
