| Cat# | DIA-192 |
| Abbr | Glucose Dehyrogenase, Native (Microorganism) |
| Source | Microorganism |
| Description | In enzymology, a quinoprotein glucose dehydrogenase (EC 1.1.5.2) is an enzyme that catalyzes the chemical reaction: D-glucose + ubiquinone ↔D-glucono-1,5-lactone + ubiquinol. Thus, the two substrates of this enzyme are D-glucose and ubiquinone, whereas its two products are D-glucono-1,5-lactone and ubiquinol. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with a quinone or similar compound as acceptor. This enzyme participates in pentose phosphate pathway. It employs one cofactor, PQQ. |
| Applications | This enzyme is useful for enzymatic determination of D-Glucose. |
| Appearance | Purple amorphous powder, lyophilized |
| Form | Freeze dried powder |
| Enzyme Commission Number | EC 1.1.5.2 |
| Activity | 500 U/mg-solid or more |
| CAS No. | 81669-60-5 |
| Contaminants | Glucose dehydrogenase < 1.0×10⁻³% (NAD-dependent); Hexokinase < 1.0×10⁻³% |
| Molecular Weight | approx. 100 kDa (by gel filtration) |
| pH Stability | pH 3.5-8.5 (25°C, 16hr) |
| Michaelis Constant | 4.8 mM (D-Glucose) |
| Optimum pH | 7 |
| Optimum temperature | 37°C |
| Thermal stability | below 50°C (pH 7.5, 30min) |
| Stability | Store at -20°C |
| Stabilizers | Ca⁺⁺, BSA |
| Inhibitors | Cu⁺⁺, Pb⁺⁺, Ag⁺ |
| Synonyms | Glucose Dehyrogenase; EC 1.1.5.2; D-glucose:ubiquinone oxidoreductase; D-glucose:(pyrroloquinoline-quinone) 1-oxidoreductase; glucose dehydrogenase (PQQ-dependent); glucose dehydrogenase (pyrroloquinoline-quinone); quinoprotein D-glucose dehydrogenase |
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