| Cat# | DIA-434 |
| Source | E. coli |
| Form | Freeze dried powder |
| Activity | ≥ 6.0 U/mg |
| Isoelectric point | 4.2 |
| Unit Definition | One unit (U) is defined as the amount of enzyme which produces 1 µmol of hydrogen peroxide per min at 37 °C and pH 8.0 under the conditions described below. |
| Storage | Below –20 °C |
| Enzyme Commission Number | EC 1.5.3 |
| pH Stability | 6.0–9.5 |
| Michaelis Constant | 3.4 × 10-3 M (Fructosyl-valyl-histidine) 4.4 × 10-3 M (Fructosyl-glycine) 8.9 × 10-3 M (Nε-fructosyl-lysine) |
| Optimum pH | 7.5–8.0 |
| Optimum temperature | 35–42 °C |
| Thermal stability | Below 45 °C |
| Contaminants | Catalase ≦ 1.0 U/U% |
| Applications | For the determination of fructosyl-peptide and fructosyl-L-amino acid. Also it is useful for the determination of HbA1c by using protease together. (HbA1c is used as a test makar to diagnose diabetes. HbA1c can also be quantified by measuring the fructosyl peptide or fructosyl-L-amino acid excised by the protease from HbA1c.) |
| Appearance | Yellow lyophilizate |
| Molecular Weight | ca. 60 kDa (Gel filtration) |
| Notes | Stable at 37 °C for at least one month |
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