| Cat# | MDE-002 |
| Specification | 100 mg, 1 g, 5 g, 10 g, 100 g, 1 kg |
| Description | Proteinase K, derived from Tritirachium album, non-specifically cleaves peptide bonds adjacent to aliphatic and aromatic amino acids. It exhibits strong proteolytic activity in DNA/RNA extraction buffers and is essential for plasmid and genomic nucleic acid isolation. |
| Applications | Suitable for the lyophilization process in molecular diagnostic kits, used for pathogen detection and nucleic acid extraction. |
| Form | White/pale yellow powder |
| Species | Tritirachium album Limber |
| Source | Bacillus subtilis |
| Purity | 95% by SDS-PAGE |
| Buffer | 20 mM Tris-HCl, 200 mM NaCl, 0.02–0.2% NaN₃, 50% glycerol, pH 8.4. After preparation, the solution must be sterilized by filtration using a 0.22 μm filter. |
| Storage | Store at 2-8 °C; if not used for an extended period, store at -20 °C. Keep lyophilized powder sealed to prevent moisture absorption. |
| Notes | Dissolve the dry powder in the recommended diluent to prepare a proteinase K solution at a concentration of 20-50 mg/mL. Sterilize the solution by filtering it through a 0.22 μm filter, then transfer it to a sterile container. If the proteinase K solution is to be used repeatedly, it must be aliquoted and stored under sterile conditions. For long-term storage, it is recommended to store the solution at -20°C. For your safety and health, please wear a lab coat, disposable gloves, and a mask during operation. It is recommended to use filter membranes made of polyethersulfone (PES) or polyvinylidene fluoride (PVDF), which have low protein-binding properties, for all sterilization processes. |
| Download Datasheet: |  |
