| Description |
RNase A is a small, stable endoribonuclease (ca. 13.7 kDa) that specifically cleaves single-stranded RNA at pyrimidine residues. It is widely used to remove RNA during DNA purification. Activity depends on salt concentration: at low salt, RNase A can cleave ssRNA, dsRNA, and RNA-DNA hybrids; at high salt, activity is restricted to ssRNA. Recommended working concentration: 1–100 μg/mL. |