diagnostic-enzymes

3α-Hydroxysteroid Dehydrogenase, Recombinant

3α-Hydroxysteroid Dehydrogenase, Recombinant

Cat# AbbrSimilarDescriptionApplicationsAppearanceFormEnzyme Commission NumberActivityMolecular WeightPurityIsoelectric pointUnit DefinitionOptimum pHActivatorsStorageBufferInhibitorsSynonyms
NATE-1138 3α-HSD, Recombinant3α-Hydroxysteroid DehydrogenaseIn enzymology, a 3alpha-hydroxysteroid dehydrogenase (B-specific) (EC 1.1.1.50) is an enzyme that catalyzes the chemical reaction: androsterone + NAD (P)+ ↔ 5alpha-androstane-3,17-dione + NAD (P)H + H+. The 3 substRates of this enzyme are androsterone, NAD+, and NADP+, whereas its 4 products are 5alpha-androstane-3,17-dione, NADH, NADPH, and H+. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor, more specifically it is part of the group of hydroxysteroid dehydrogenases.Bile acid is one of the substrates of 3α-hydroxy steroiddehydrogenase. 3α-hydroxy steroid dehydrogenase is used to catalyzethe dehydrogenation reaction of hydroxy steroid in clinic. So, HSD is used to detect the total bile acid clinically.White powder, lyophilizedFreeze dried powderEC 1.1.1.50About 50U/mg powderAbout 28 kDa (SDS-PAGE detection)90% (SDS-PAGE test)4.8One unit will catalyze the oxidation of 1µmol of androsterone per min at pH8.9 at 25°C.7.0-9.0EDTA4°C, store at -20°C for long-term preservation.20mM Tris, pH8.0Hg2+, Ag+hydroxyprostaglandin dehydrogenase; 3α-hydroxysteroid oxidoreductase; sterognost 3α; 3α-hydroxysteroid dehydrogenase (B-specific); 3α-hydroxysteroid 3-dehydrogenase (B-specific); 3α-hydroxysteroid:NAD (P)+ 3-oxidoreductase (B-specific); EC 1.1.1.50 Download Datasheet:
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