Cholesterol Oxidase from Streptomyces sp.
| Cat# | NATE-0128 |
| Source | Streptomyces sp. |
| Description | Cholesterol oxidase (CHOD) is a monomeric flavoprotein containing FAD that catalyzes the first step in cholesterol catabolism. This bifunctional enzyme oxidizes cholesterol to cholest-5-en-3-one in an FAD-requiring step, which is then isomerized to cholest-4-en-3-one with the release of H2O2. |
| Form | Freeze dried powder |
| Enzyme Commission Number | EC 1.1.3.6 |
| Activity | 15U/mg-solid or more |
| CAS No. | 9028-76-6 |
| Isoelectric point | 5.1 ± 0.1 and 5.4 ± 0.1 |
| pH Stability | pH 5.0 – 10.0 (25°C, 20hr) |
| Michaelis Constant | 4.3 x 10‾5M (Cholesterol) |
| Unit Definition | One unit will convert 1.0 μmole of cholesterol to 4-cholesten-3-one per min at pH 7.5 at 25°C. Note: 4-cholesten-3-one may undergo isomerization. |
| Optimum pH | 6.5 − 7.0 |
| Optimum temperature | 45 – 50°C |
| Thermal stability | Below 45°C (pH 7.0, 15min) |
| Storage | −20°C |
| Buffer | 50 mM potassium phosphate buffer, pH 7.0: soluble (Cold) |
| Inhibitors | Ionic detergents, Hg++, Ag+ |
| Synonyms | EC 1.1.3.6, cholesterol-O2 oxidoreductase; 3β-hydroxy steroid oxidoreductase; 3β-hydroxysteroid:oxygen oxidoreductase; 9028-76-6 |
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