Native Cystathionine-β-lyase (CBL) from Microorganism
Cat#
BDE-020
Specification
5 KU, 50 KU, 500 KU
Description
Cystathionine-β-lyase (CBL) cleaves cystathionine to produce cysteine and plays a central role in hydrogen sulfide production and amino acid metabolism.
Applications
Used in the coenzyme system for homocysteine detection to achieve cascading signal amplification.
Enzyme Commission Number
EC 4.4.1.8
Form
Yellow amorphous lyophilized powder
Source
Microorganism
Activity
≥ 30 U/mg
Unit Definition
One unit is defined as the amount of enzyme required to consume 1 μmol of NADH per minute under specified assay conditions.
Molecular Weight
54 kDa (SDS-PAGE)
Purity
≥ 90%
Optimum pH
8.0
Optimum temperature
50°C
pH Stability
6.0-10.0 (25°C, 16 h)
Thermal Stability
< 45°C (pH 8.0, 30 min)
Isoelectric Point
6.0
Km Value
1.6×10^-3 M (L-Cystathionine)
Inhibitors
Not inhibited by NaN3
Protectant
Triton X-100, glycerol
Storage
Store at -25°C to -15°C for 12 months to maintain at least 90% activity.