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Native Cystathionine-β-lyase (CBL) from Microorganism

Cat# BDE-020
Specification 5 KU, 50 KU, 500 KU
Description Cystathionine-β-lyase (CBL) cleaves cystathionine to produce cysteine and plays a central role in hydrogen sulfide production and amino acid metabolism.
Applications Used in the coenzyme system for homocysteine detection to achieve cascading signal amplification.
Enzyme Commission Number EC 4.4.1.8
Form Yellow amorphous lyophilized powder
Source Microorganism
Activity ≥ 30 U/mg
Unit Definition One unit is defined as the amount of enzyme required to consume 1 μmol of NADH per minute under specified assay conditions.
Molecular Weight 54 kDa (SDS-PAGE)
Purity ≥ 90%
Optimum pH 8.0
Optimum temperature 50°C
pH Stability 6.0-10.0 (25°C, 16 h)
Thermal Stability < 45°C (pH 8.0, 30 min)
Isoelectric Point 6.0
Km Value 1.6×10^-3 M (L-Cystathionine)
Inhibitors Not inhibited by NaN3
Protectant Triton X-100, glycerol
Storage Store at -25°C to -15°C for 12 months to maintain at least 90% activity.
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