| Cat# | TRA-004 |
| Specification | 5KU |
| Description | Pyruvate kinase converts phosphoenolpyruvate and ADP into ATP and pyruvate, serving as one of the primary rate-limiting enzymes in glycolysis. It possesses two isoenzymes, M-type and L-type, with the M-type further subdivided into M1 and M2 subtypes. |
| CAS No. | 9001-59-6 |
| Enzyme Commission Number | EC 2.7.1.40 |
| Synonyms | PK |
| Form | White amorphous powder |
| Source | Microorganism |
| Activity | ≥ 300 U/mg |
| Unit Definition | One unit will convert 1 μmol of phosphoenolpyruvate to pyruvate per min at pH 7.2 at 30°C. |
| Molecular Weight | 68 kDa (SDS-PAGE) |
| Optimum pH | 7.5 |
| Optimum temperature | 65°C |
| pH Stability | 5.0-10.0 (37°C, 20 h) |
| Thermal Stability | < 60°C (pH 8.5, 20 min) |
| Isoelectric Point | 5.2 |
| Km Value | 1.1×10 ^-3 M (ADP), 2.2×10^-3 M (PEP) |
| Inhibitors | Ca2+, Co2+, Cu2+, Fe3+, Mn2+, Ni2+, NEM, Proclin, SDS |
| Storage | -20°C |
| Download Datasheet: |  |
