Glycerokinase from Microorganism
Glycerokinase from Microorganism

Glycerokinase from Microorganism

Cat# DIA-149
Abbr GK (Microorganism)
Alias GK
Source Microorganism
Description Glycerol kinase is a phosphotransferase enzyme involved in triglycerides and glycerophospholipids synthesis. Glycerol kinase catalyzes tge MgATP-dependent phosphorylation of glycerol to produce sn-glycerol-3-phosphate and is the rate limiting enzyme in the utilization of glycerol. It is also subject to feedback regulation by fructose-1,6-bisphosphate.
Applications This enzyme is useful for enzymatic determination of glycerol and triglyceride when coupled with glycerol-3-phosphate dehydrogenase, glycerol-3-phosphate oxidase or pyruvate kinase and lactate dehydrogenase, lipoprotein lipase in clinical analysis.
Appearance White amorphous powder, lyophilized
Form Freeze dried powder
Enzyme Commission Number EC 2.7.1.30
Activity 30 U/mg-solid or more
CAS No. 9030-66-4
Contaminants Catalase < 1.0×10⁻¹% NADH oxidase < 1.0×10⁻³% Adenosine triphosphatase < 1.0×10⁻³%
Isoelectric point 4.3
pH Stability pH 5.5-10.0 (25°C, 20hr)
Michaelis Constant 9.4×10⁻⁵M (Glycerol), 1.3×10⁻⁵M (ATP), 2.1×10⁻³M (Dihydroxyacetone)
Structure Four subunits of approx. 58,000
Optimum pH 10
Optimum temperature 70°
Thermal stability below 65°C (pH 7.5, 30min)
Stability Stable at-20°C
Inhibitors p-Chloromercuribenzoate, Hg⁺⁺, Ag⁺
Synonyms glycerokinase; GK; ATP: glycerol-3-phosphotransferase; glycerol kinase phosphorylating; glyceric kinase; EC 2.7.1.30
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