| Cat# | DIA-149 |
| Abbr | GK (Microorganism) |
| Alias | GK |
| Source | Microorganism |
| Description | Glycerol kinase is a phosphotransferase enzyme involved in triglycerides and glycerophospholipids synthesis. Glycerol kinase catalyzes tge MgATP-dependent phosphorylation of glycerol to produce sn-glycerol-3-phosphate and is the rate limiting enzyme in the utilization of glycerol. It is also subject to feedback regulation by fructose-1,6-bisphosphate. |
| Applications | This enzyme is useful for enzymatic determination of glycerol and triglyceride when coupled with glycerol-3-phosphate dehydrogenase, glycerol-3-phosphate oxidase or pyruvate kinase and lactate dehydrogenase, lipoprotein lipase in clinical analysis. |
| Appearance | White amorphous powder, lyophilized |
| Form | Freeze dried powder |
| Enzyme Commission Number | EC 2.7.1.30 |
| Activity | 30 U/mg-solid or more |
| CAS No. | 9030-66-4 |
| Contaminants | Catalase < 1.0×10⁻¹% NADH oxidase < 1.0×10⁻³% Adenosine triphosphatase < 1.0×10⁻³% |
| Isoelectric point | 4.3 |
| pH Stability | pH 5.5-10.0 (25°C, 20hr) |
| Michaelis Constant | 9.4×10⁻⁵M (Glycerol), 1.3×10⁻⁵M (ATP), 2.1×10⁻³M (Dihydroxyacetone) |
| Structure | Four subunits of approx. 58,000 |
| Optimum pH | 10 |
| Optimum temperature | 70° |
| Thermal stability | below 65°C (pH 7.5, 30min) |
| Stability | Stable at-20°C |
| Inhibitors | p-Chloromercuribenzoate, Hg⁺⁺, Ag⁺ |
| Synonyms | glycerokinase; GK; ATP: glycerol-3-phosphotransferase; glycerol kinase phosphorylating; glyceric kinase; EC 2.7.1.30 |
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