| Cat# | ENZD-MDLyo02 |
| Specification | 10000 U; 40000 U |
| Unit Concentration | 200 U/μl |
| Description | High Reverse Transcriptase II (Glycerol-free) is a lyophilizable formulation of EnzDiagnostic's High Reverse Transcriptase II. By removing glycerol, this product combines the convenience of lyophilization with the enzyme's inherent performance and stability, making it ideal for self-built one-step systems. |
| Notes | Highly stable reverse transcriptase, optimized for lyophilization and one-step systems. |
| Features | • High stability: Exhibits high reagent stability. • Compatible with self-built one-step systems: Demonstrates high reverse transcription performance in self-built one-step systems (qRT-PCR premix). |
| Applications | Lyophilized reverse transcription reagent for one-step RT-qPCR. |
| Components | 5 x HiScript II Buffer HiScript II Reverse Transcriptase (Glycerol-free) (200 U/µl) |
| Quality Control | High stability; Adaptable to self-built one-step method system |
| Shipping and Storage Conditions | Store at -30 ~ -15℃, transport at ≤0℃. |
| Download Datasheet: |  |
Using 100 ng/μL HeLa total RNA as template, reverse transcription was performed with ENZD-MDLyo02 from Creative Enzymes. The resulting cDNA was evaluated by qPCR across four independent reaction systems (T28, T29, T76, T78) to assess performance consistency under different conditions.
Results showed that ENZD-MDLyo02 maintained highly stable performance across freeze-dried, batch-to-batch, and thermal stability tests. The ΔCT values between treated and untreated samples remained within ±0.5, indicating minimal performance variation. These findings demonstrate excellent stability and reproducibility of the enzyme under multiple processing conditions.
As a lyophilization-compatible enzyme, ENZD-MDLyo02 ensures reliable reverse transcription performance even after formulation or environmental stress. Data generated from internal validation studies, confirming high stability and consistent performance across multiple test conditions.
The performance of ENZD-MDLyo02 was evaluated in a self-developed one-step amplification system using mixed RNA templates, including H1N1, influenza B, RSV, and HeLa RNA at multiple concentration levels.
Across all targets (20,000/2,000/200 copies/mL for H1N1 and influenza B; 50,000/5,000/500 copies/mL for RSV), all replicates were consistently detected with a 100% detection rate. These results demonstrate strong reverse transcription efficiency and excellent template compatibility in complex multiplex systems.
As a lyophilization-compatible reverse transcriptase, ENZD-MDLyo02 supports robust performance in one-step diagnostic workflows with high sensitivity and reproducibility. Data generated from internal validation studies, demonstrating reliable detection performance in multiplex one-step amplification systems.
