Native Microorganism Glutamate Dehydrogenase (NAD-dependent)
| Cat# | DIA-197 |
| Abbr | GLDH (NAD) (Microorganism) |
| Alias | GLDH |
| Source | Microorganism |
| Description | Glutamate dehydrogenase (GLDH) is an enzyme, present in most microbes and the mitochondria of eukaryotes, as are some of the other enzymes required for urea synthesis, that converts glutamate to α-ketoglutarate, and vice versa. In animals, the produced ammonia is usually used as a substrate in the urea cycle. Typically, the α-ketoglutarate to glutamate reaction does not occur in mammals, as glutamate dehydrogenase equilibrium favours the production of ammonia and α-ketoglutarate. |
| Applications | This enzyme is useful for enzymatic determination of NH3, α-ketoglutaric acid and L-glutamic acid, and for assay of leucine aminopeptidase and urease. This enzyme is also used for enzymatic determination of urea when coupled with urease in clinical analysis. |
| Appearance | White amorphous powder, lyophilized |
| Form | Freeze dried powder |
| Enzyme Commission Number | EC 1.4.1.2 |
| Activity | 100 U/mg-solid or more |
| CAS No. | 9001-46-1 |
| Contaminants | NAD oxidase < 1.0×10⁻²% |
| Molecular Weight | approx. 260 kDa |
| Isoelectric point | 5.6 |
| pH Stability | pH 5.0-10.0 (25°C, 20hr) |
| Michaelis Constant | 9.21×10⁻³M (NH₃), 4.80×10⁻³M (α-Ketoglutarate), 7.8×10⁻⁵M (L-Glutamate), 1.29×10⁻⁴M (NADH), 5.89×10⁻⁴M (NAD⁺) |
| Structure | 6 subunits per mol of enzyme |
| Optimum pH | 7.5-8.0 (α-KG→L-Glu) 9.0 (L-Glu→α-KG) |
| Optimum temperature | 55°C (α-KG→L-Glu) 50°C (L-Glu→α-KG) |
| Thermal stability | below 50°C (pH 8.3, 10min) |
| Stability | Stable at-20°C for at least one year |
| Inhibitors | Heavy metals, PCMB, IAA |
| Synonyms | Glutamate Dehydrogenase; glutamic dehydrogenase; glutamate dehydrogenase (NAD); glutamate oxidoreductase; glutamic acid dehydrogenase; L-glutamate dehydrogenase; NAD-dependent glutamate dehydrogenase; NAD-dependent glutamic dehydrogenase; NAD-glutamate dehydrogenase; NAD-linked glutamate dehydrogenase; NAD-linked glutamic dehydrogenase; NAD-specific glutamic dehydrogenase; NAD-specific glutamate dehydrogenase; NAD: glutamate oxidoreductase; NADH-linked glutamate dehydrogenase; L-glutamate: NAD+ oxidoreductase (deaminating); EC 1.4.1.2; GLDH |
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