diagnostic-enzymes

Native Microorganism Glutamate Dehydrogenase (NAD-dependent)

Native Microorganism Glutamate Dehydrogenase (NAD-dependent)

Cat# AbbrAliasSimilarSourceDescriptionApplicationsAppearanceFormEnzyme Commission NumberActivityCAS No. ContaminantsMolecular WeightIsoelectric pointpH StabilityMichaelis ConstantStructureOptimum pHOptimum temperatureThermal stabilityStabilityInhibitorsSynonyms
DIA-197 GLDH (NAD) (Microorganism)GLDHGLDHMicroorganismGlutamate dehydrogenase (GLDH) is an enzyme, present in most microbes and the mitochondria of eukaryotes, as are some of the other enzymes required for urea synthesis, that converts glutamate to α-ketoglutarate, and vice versa. In animals, the produced ammonia is usually used as a substrate in the urea cycle. Typically, the α-ketoglutarate to glutamate reaction does not occur in mammals, as glutamate dehydrogenase equilibrium favours the production of ammonia and α-ketoglutarate.This enzyme is useful for enzymatic determination of NH3, α-ketoglutaric acid and L-glutamic acid, and for assay of leucine aminopeptidase and urease. This enzyme is also used for enzymatic determination of urea when coupled with urease in clinical analysis.White amorphous powder, lyophilizedFreeze dried powderEC 1.4.1.2100 U/mg-solid or more9001-46-1NAD oxidase < 1.0×10⁻²%approx. 260 kDa5.6pH 5.0-10.0 (25°C, 20hr)9.21×10⁻³M (NH₃), 4.80×10⁻³M (α-Ketoglutarate), 7.8×10⁻⁵M (L-Glutamate), 1.29×10⁻⁴M (NADH), 5.89×10⁻⁴M (NAD⁺)6 subunits per mol of enzyme7.5-8.0 (α-KG→L-Glu) 9.0 (L-Glu→α-KG)55°C (α-KG→L-Glu) 50°C (L-Glu→α-KG)below 50°C (pH 8.3, 10min)Stable at-20°C for at least one yearHeavy metals, PCMB, IAAGlutamate Dehydrogenase; glutamic dehydrogenase; glutamate dehydrogenase (NAD); glutamate oxidoreductase; glutamic acid dehydrogenase; L-glutamate dehydrogenase; NAD-dependent glutamate dehydrogenase; NAD-dependent glutamic dehydrogenase; NAD-glutamate dehydrogenase; NAD-linked glutamate dehydrogenase; NAD-linked glutamic dehydrogenase; NAD-specific glutamic dehydrogenase; NAD-specific glutamate dehydrogenase; NAD: glutamate oxidoreductase; NADH-linked glutamate dehydrogenase; L-glutamate: NAD+ oxidoreductase (deaminating); EC 1.4.1.2; GLDH Download Datasheet:
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