Native Microorganism Glutamate Dehydrogenase (NAD-dependent)
Native Microorganism Glutamate Dehydrogenase (NAD-dependent)

Native Microorganism Glutamate Dehydrogenase (NAD-dependent)

Cat# DIA-197
Abbr GLDH (NAD) (Microorganism)
Alias GLDH
Source Microorganism
Description Glutamate dehydrogenase (GLDH) is an enzyme, present in most microbes and the mitochondria of eukaryotes, as are some of the other enzymes required for urea synthesis, that converts glutamate to α-ketoglutarate, and vice versa. In animals, the produced ammonia is usually used as a substrate in the urea cycle. Typically, the α-ketoglutarate to glutamate reaction does not occur in mammals, as glutamate dehydrogenase equilibrium favours the production of ammonia and α-ketoglutarate.
Applications This enzyme is useful for enzymatic determination of NH3, α-ketoglutaric acid and L-glutamic acid, and for assay of leucine aminopeptidase and urease. This enzyme is also used for enzymatic determination of urea when coupled with urease in clinical analysis.
Appearance White amorphous powder, lyophilized
Form Freeze dried powder
Enzyme Commission Number EC 1.4.1.2
Activity 100 U/mg-solid or more
CAS No. 9001-46-1
Contaminants NAD oxidase < 1.0×10⁻²%
Molecular Weight approx. 260 kDa
Isoelectric point 5.6
pH Stability pH 5.0-10.0 (25°C, 20hr)
Michaelis Constant 9.21×10⁻³M (NH₃), 4.80×10⁻³M (α-Ketoglutarate), 7.8×10⁻⁵M (L-Glutamate), 1.29×10⁻⁴M (NADH), 5.89×10⁻⁴M (NAD⁺)
Structure 6 subunits per mol of enzyme
Optimum pH 7.5-8.0 (α-KG→L-Glu) 9.0 (L-Glu→α-KG)
Optimum temperature 55°C (α-KG→L-Glu) 50°C (L-Glu→α-KG)
Thermal stability below 50°C (pH 8.3, 10min)
Stability Stable at-20°C for at least one year
Inhibitors Heavy metals, PCMB, IAA
Synonyms Glutamate Dehydrogenase; glutamic dehydrogenase; glutamate dehydrogenase (NAD); glutamate oxidoreductase; glutamic acid dehydrogenase; L-glutamate dehydrogenase; NAD-dependent glutamate dehydrogenase; NAD-dependent glutamic dehydrogenase; NAD-glutamate dehydrogenase; NAD-linked glutamate dehydrogenase; NAD-linked glutamic dehydrogenase; NAD-specific glutamic dehydrogenase; NAD-specific glutamate dehydrogenase; NAD: glutamate oxidoreductase; NADH-linked glutamate dehydrogenase; L-glutamate: NAD+ oxidoreductase (deaminating); EC 1.4.1.2; GLDH
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